| pubmed-article:2634134 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2634134 | lifeskim:mentions | umls-concept:C0370231 | lld:lifeskim |
| pubmed-article:2634134 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:2634134 | lifeskim:mentions | umls-concept:C0185115 | lld:lifeskim |
| pubmed-article:2634134 | lifeskim:mentions | umls-concept:C1553035 | lld:lifeskim |
| pubmed-article:2634134 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2634134 | pubmed:dateCreated | 1990-5-30 | lld:pubmed |
| pubmed-article:2634134 | pubmed:abstractText | We describe methods for extracting genomic DNA from a small amount of whole blood or cultured amniocytes. Nuclear DNA was extracted from whole blood spotted on blotting paper. Relatively large molecules of DNA with the average amount of 7-9 micrograms was extracted from 1 ml of blood spotted and stored for at most two years, being roughly 1/3 of that extracted directly from fresh whole blood. The estimated minimum amount of whole blood that gives a suitable autoradiogram of Southern hybridization was 0.3 ml. Another series of amounts of whole blood or an amniocyte suspension were molded in low-melting agarose into an 100 microliter gel block. The DNA extracted from a block that was made from at least 0.25 ml of whole blood, or from 1.25 x 10(5) amniocytes (equivalent to 1/8 of the number of confluent cells in a 25 cm2 culture flask) resulted in one suitable Southern analysis. Both methods described here are applicable to the diagnosis of newborns and/or fetuses at risk of a genetic disease and to the diagnosis of a patient from whom a large amount of blood material is difficult to obtain. These methods also make a long-way transportation of the materials possible. | lld:pubmed |
| pubmed-article:2634134 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2634134 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2634134 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2634134 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2634134 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2634134 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2634134 | pubmed:issn | 0021-5074 | lld:pubmed |
| pubmed-article:2634134 | pubmed:author | pubmed-author:MatsumotoTT | lld:pubmed |
| pubmed-article:2634134 | pubmed:author | pubmed-author:NiikawaNN | lld:pubmed |
| pubmed-article:2634134 | pubmed:author | pubmed-author:HirotaTT | lld:pubmed |
| pubmed-article:2634134 | pubmed:author | pubmed-author:KondohTT | lld:pubmed |
| pubmed-article:2634134 | pubmed:author | pubmed-author:JinnoYY | lld:pubmed |
| pubmed-article:2634134 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2634134 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:2634134 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2634134 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2634134 | pubmed:pagination | 217-23 | lld:pubmed |
| pubmed-article:2634134 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:meshHeading | pubmed-meshheading:2634134-... | lld:pubmed |
| pubmed-article:2634134 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2634134 | pubmed:articleTitle | Micro extraction of DNA from whole blood and amniocytes. | lld:pubmed |
| pubmed-article:2634134 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2634134 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
