| pubmed-article:2585554 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C1518683 | lld:lifeskim |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C1510470 | lld:lifeskim |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C0003233 | lld:lifeskim |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C1550548 | lld:lifeskim |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C1555714 | lld:lifeskim |
| pubmed-article:2585554 | lifeskim:mentions | umls-concept:C1705654 | lld:lifeskim |
| pubmed-article:2585554 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2585554 | pubmed:dateCreated | 1989-12-28 | lld:pubmed |
| pubmed-article:2585554 | pubmed:abstractText | Cochlear outer hair cells have been well established as primary targets of the ototoxic actions of aminoglycoside antibiotics. These cells, isolated from the guinea pig cochlea and maintained in short-term culture, were used as a model for evaluating the acute effects of gentamicin on cell viability, depolarization-induced transmembrane calcium flux, and depolarization-induced motile responses. On the basis of morphology and fluorochromasia, the presence of extracellular gentamicin as high as 5 mM did not affect the viability of the cells for up to 6 hr, the longest time tested. Viable cells showed binding of fluorescently tagged gentamicin to their base but excluded the drug from their cytoplasm. In response to [K+]-depolarization, intracellular calcium levels (monitored with the fluorescent calcium-sensitive dye fluo-3) increased from a resting value of 218 +/- 102 nM to 2,018 +/- 1,077 nM concomitant with a cell shortening of 0.7% +/- 1.3%. The depolarization-induced calcium increase was apparently caused by calcium entry into the cell as it was inhibited by the calcium-channel blocker methoxyverapamil and prevented in the absence of extracellular calcium. Both gentamicin and neomycin blocked the [K+]-induced calcium increase at an IC50 of 50 microM. Despite the inhibition of calcium entry the ability of the outer hair cells to shorten under [K+]-depolarization was not impaired; in fact, cell shortening was even more pronounced in the absence of calcium influx (2.6% +/- 1.4%). This argues effectively against the existence of a calcium-dependent actomyosin-mediated component in [K+]-induced shape changes.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:2585554 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2585554 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2585554 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2585554 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2585554 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2585554 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2585554 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2585554 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2585554 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2585554 | pubmed:issn | 0360-4012 | lld:pubmed |
| pubmed-article:2585554 | pubmed:author | pubmed-author:AranJ MJM | lld:pubmed |
| pubmed-article:2585554 | pubmed:author | pubmed-author:SchachtJJ | lld:pubmed |
| pubmed-article:2585554 | pubmed:author | pubmed-author:ZajicGG | lld:pubmed |
| pubmed-article:2585554 | pubmed:author | pubmed-author:DulonDD | lld:pubmed |
| pubmed-article:2585554 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2585554 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:2585554 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2585554 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2585554 | pubmed:pagination | 338-46 | lld:pubmed |
| pubmed-article:2585554 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:2585554 | pubmed:meshHeading | pubmed-meshheading:2585554-... | lld:pubmed |
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| pubmed-article:2585554 | pubmed:meshHeading | pubmed-meshheading:2585554-... | lld:pubmed |
| pubmed-article:2585554 | pubmed:meshHeading | pubmed-meshheading:2585554-... | lld:pubmed |
| pubmed-article:2585554 | pubmed:meshHeading | pubmed-meshheading:2585554-... | lld:pubmed |
| pubmed-article:2585554 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2585554 | pubmed:articleTitle | Aminoglycoside antibiotics impair calcium entry but not viability and motility in isolated cochlear outer hair cells. | lld:pubmed |
| pubmed-article:2585554 | pubmed:affiliation | Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109-0506. | lld:pubmed |
| pubmed-article:2585554 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2585554 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:2585554 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2585554 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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