| pubmed-article:2553131 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0338106 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C1510779 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0311400 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0205615 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0205618 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0024487 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:2553131 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:2553131 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2553131 | pubmed:dateCreated | 1989-12-12 | lld:pubmed |
| pubmed-article:2553131 | pubmed:abstractText | Aspects of energetic and intermediary metabolism were studied in a colon adenocarcinoma cell line (HT29) by multinuclear magnetic resonance spectroscopy. Experiments were carried out on the HT29-D4 clone, which was isolated by limit dilution techniques. This clone, usually undifferentiated (D4-UD), can be maintained in a differentiated state (D4-D) in a glucose-free medium. Metabolic data were obtained by NMR analysis of perchloric acid extracts from D4-UD and D4-D cells. Phosphorus-31 and proton NMR spectra showed the presence of a large amount of choline and phosphorylcholine in the differentiated state (400% and 200%, respectively, of the levels found in D4-UD cells). Other differences appeared in the content of phosphocreatine (absent in D4-D cells) and myoinositol (absent in D4-UD cells). Carbon-13 spectra were recorded from perchloric acid extracts of cells incubated with [1-13C]-labeled glucose or [2-13C]-labeled acetate. The data indicated that both types of cells metabolize glucose through the glycolytic pathway to give lactate, but only D4-D cells were able to store glucose as glycogen at a very high level. A mathematical analysis of fluxes through the tricarboxylic acid (TCA) cycle was developed on the basis of models derived from previous 14C tracer studies. The model was based on the steady-state labeling of glutamate carbons by the 13C isotope and gave the fraction of labeled acetyl-Coa entering the TCA cycle, and the activity y of anaplerotic reactions relative to the flux through the citrate synthetase reaction. The data indicated that y greater than 0.3 in all cases. Only 15% and 30% of labeled acetyl CoA entered the TCA cycle in D4-UD and D4-D cells, respectively, under labeled glucose incubation: these values were significantly different upon labeled acetate feeding, reaching 55% for D4-UD cells and 85% for D4-D cells. The main result of this study is that the process of differentiation of HT29 cells is correlated with a large increase in the activity of oxidative metabolism. | lld:pubmed |
| pubmed-article:2553131 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2553131 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2553131 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2553131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2553131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2553131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2553131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2553131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2553131 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2553131 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2553131 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2553131 | pubmed:issn | 0300-9084 | lld:pubmed |
| pubmed-article:2553131 | pubmed:author | pubmed-author:CozzoneP JPJ | lld:pubmed |
| pubmed-article:2553131 | pubmed:author | pubmed-author:CanioniPP | lld:pubmed |
| pubmed-article:2553131 | pubmed:author | pubmed-author:FantiniJJ | lld:pubmed |
| pubmed-article:2553131 | pubmed:author | pubmed-author:Vion-DuryJJ | lld:pubmed |
| pubmed-article:2553131 | pubmed:author | pubmed-author:GalonsJ PJP | lld:pubmed |
| pubmed-article:2553131 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2553131 | pubmed:volume | 71 | lld:pubmed |
| pubmed-article:2553131 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2553131 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2553131 | pubmed:pagination | 949-61 | lld:pubmed |
| pubmed-article:2553131 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2553131 | pubmed:meshHeading | pubmed-meshheading:2553131-... | lld:pubmed |
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| pubmed-article:2553131 | pubmed:meshHeading | pubmed-meshheading:2553131-... | lld:pubmed |
| pubmed-article:2553131 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2553131 | pubmed:articleTitle | Metabolic changes in undifferentiated and differentiated human colon adenocarcinoma cells studied by multinuclear magnetic resonance spectroscopy. | lld:pubmed |
| pubmed-article:2553131 | pubmed:affiliation | Centre de Résonance Magnétique Biologique et Médicale, URA CNRS, Faculté de Médecine, Marseille, France. | lld:pubmed |
| pubmed-article:2553131 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2553131 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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