| pubmed-article:2549979 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C1882727 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C0008286 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C0085862 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C1299583 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C2263450 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C2346927 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C1608386 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C1549571 | lld:lifeskim |
| pubmed-article:2549979 | lifeskim:mentions | umls-concept:C0146951 | lld:lifeskim |
| pubmed-article:2549979 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2549979 | pubmed:dateCreated | 1989-10-12 | lld:pubmed |
| pubmed-article:2549979 | pubmed:abstractText | Lung contains both Mg2+-dependent and Mg2+-independent phosphatidate phosphohydrolase activities. Addition of Triton X-100 (0.5%) or chlorpromazine (1 mM) leads to a marked increase in the total phosphatidate phosphohydrolase activity in rat lung microsomes (microsomal fractions), but a decrease in the Mg2+-dependent activity. These observations suggest that the Mg2+-independent activity is stimulated, whereas the Mg2+-dependent activity is inhibited. However, the possibility exists that Triton X-100 could stimulate the Mg2+-dependent enzymic activity in an Mg2+-independent manner. In addition, the positively charged amphiphilic drug could be replacing the enzyme's requirement for Mg2+. These two possibilities were examined by using subcellular fractions in which the Mg2+-dependent phosphatidate phosphohydrolase had been abolished by heat treatment at 55 degrees C for 15 min. Heat treatment does not affect the microsomal Mg2+-independent phosphohydrolase to any great extent. Since the 6-8-fold stimulations due to Triton X-100 and chlorpromazine are retained after heat treatment of this fraction, the Mg2+-independent activity must be involved. Addition of Triton X-100 and chlorpromazine to cytosol virtually abolishes the Mg2+-dependent phosphatidate phosphohydrolase activity and decreases the Mg2+-independent activity by half. Heat treatment also abolishes the Mg2+-dependent activity and decreases the Mg2+-independent activity by over half. The Mg2+-independent phosphatidate phosphohydrolase activity remaining after heat treatment was not affected by Triton X-100 or chlorpromazine. These studies demonstrate that Triton X-100 and chlorpromazine specifically stimulate the heat-stable Mg2+-independent phosphatidate phosphohydrolase activity in rat lung microsomes. In contrast, the heat-labile Mg2+-independent phosphatidate phosphohydrolase activities in cytosol are inhibited by these reagents. Triton X-100 and chlorpromazine inhibit the Mg2+-dependent phosphatidate phosphohydrolase activities in both rat lung microsomes and cytosol. These results are consistent with the view that a single Mg2+-dependent phosphatidate phosphohydrolase present in both microsomes and cytosol is specifically involved in glycerolipid metabolism. | lld:pubmed |
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| pubmed-article:2549979 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2549979 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2549979 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2549979 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2549979 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2549979 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:2549979 | pubmed:author | pubmed-author:PossmayerFF | lld:pubmed |
| pubmed-article:2549979 | pubmed:author | pubmed-author:WaltonP APA | lld:pubmed |
| pubmed-article:2549979 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2549979 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2549979 | pubmed:volume | 261 | lld:pubmed |
| pubmed-article:2549979 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2549979 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2549979 | pubmed:pagination | 673-8 | lld:pubmed |
| pubmed-article:2549979 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2549979 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2549979 | pubmed:articleTitle | The effects of Triton X-100 and chlorpromazine on the Mg2+-dependent and Mg2+-independent phosphatidate phosphohydrolase activities of rat lung. | lld:pubmed |
| pubmed-article:2549979 | pubmed:affiliation | Department of Biochemistry, University of Western Ontario, London, Canada. | lld:pubmed |
| pubmed-article:2549979 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2549979 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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