| pubmed-article:2545497 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2545497 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:2545497 | lifeskim:mentions | umls-concept:C0010531 | lld:lifeskim |
| pubmed-article:2545497 | lifeskim:mentions | umls-concept:C0001492 | lld:lifeskim |
| pubmed-article:2545497 | lifeskim:mentions | umls-concept:C0178702 | lld:lifeskim |
| pubmed-article:2545497 | lifeskim:mentions | umls-concept:C0883208 | lld:lifeskim |
| pubmed-article:2545497 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:2545497 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:2545497 | pubmed:dateCreated | 1989-8-11 | lld:pubmed |
| pubmed-article:2545497 | pubmed:abstractText | We have examined the cell-free heterologous desensitization of adenylyl cyclase in plasma membrane preparations from S49 wild-type (WT) and kin- cells (which lack cAMP-dependent protein kinase) incubated with purified catalytic subunit of cAMP-dependent protein kinase (cA.PKc). cA.PKc caused a rapid (t1/2 = 40 s) decrease in the hormone responsiveness of adenylyl cyclase in the WT membrane preparations that mimicked the intact cell heterologous desensitization; that is, there was an increase in the Kact for both epinephrine and prostaglandin E1 (PGE1) stimulations of adenylyl cyclase induced at the receptor level because neither forskolin- nor NaF-stimulated activity was affected. The desensitization was independent of agonist occupancy of the receptor, and the effects were blocked both by the active fragment (amino acids 5-22) of the specific inhibitor of cA.PK and by p[NH]ppA. cA.PKc treatment of kin- membranes resulted in a heterologous desensitization that resembled the effects of WT adenylyl cyclase, with the exception that forskolin-stimulated activity was also reproducibly decreased by 24%. cA.PKc had no effect on WT membranes isolated from cells that had previously undergone maximal heterologous desensitization during treatment with 10 microM forskolin. In contrast, cA.PKc-induced heterologous desensitization of kin- membranes was additive with the epinephrine-induced homologous desensitization of intact cells. Cell-free desensitizations were reversed by incubation of membranes with cA.PKc and ADP, conditions that drive the kinase reaction backward. The similarities of our cell-free cA.PKc-mediated heterologous desensitization of adenylyl cyclase with the intact cell desensitization support our hypothesis that heterologous desensitization of the WT lymphoma cells is mediated by cA.PK via a mechanism independent of homologous desensitization. | lld:pubmed |
| pubmed-article:2545497 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2545497 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2545497 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2545497 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2545497 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2545497 | pubmed:issn | 0892-6638 | lld:pubmed |
| pubmed-article:2545497 | pubmed:author | pubmed-author:ClarkR BRB | lld:pubmed |
| pubmed-article:2545497 | pubmed:author | pubmed-author:FriedmanJJ | lld:pubmed |
| pubmed-article:2545497 | pubmed:author | pubmed-author:ShenolikarSS | lld:pubmed |
| pubmed-article:2545497 | pubmed:author | pubmed-author:KunkelM WMW | lld:pubmed |
| pubmed-article:2545497 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2545497 | pubmed:volume | 3 | lld:pubmed |
| pubmed-article:2545497 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2545497 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2545497 | pubmed:pagination | 2067-74 | lld:pubmed |
| pubmed-article:2545497 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
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| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:meshHeading | pubmed-meshheading:2545497-... | lld:pubmed |
| pubmed-article:2545497 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2545497 | pubmed:articleTitle | Cell-free heterologous desensitization of adenylyl cyclase in S49 lymphoma cell membranes mediated by cAMP-dependent protein kinase. | lld:pubmed |
| pubmed-article:2545497 | pubmed:affiliation | Graduate School of Biomedical Sciences, University of Texas Health Science Center, Houston 77225. | lld:pubmed |
| pubmed-article:2545497 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2545497 | pubmed:publicationType | In Vitro | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2545497 | lld:pubmed |
