| pubmed-article:2543652 | pubmed:abstractText | Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1-2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with [111In]In acetate to a specific activity of 37-74 kBq/micrograms (1-2 microCi/micrograms). The radiochemical purity of the antibody preparation was 76-81%. The immunoreactive fraction of the radiolabelled antibodies ranged from 0.17 to 0.31, significantly lower than that of their radioiodinated analogs. | lld:pubmed |
