| pubmed-article:2541616 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2541616 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2541616 | lifeskim:mentions | umls-concept:C0026339 | lld:lifeskim |
| pubmed-article:2541616 | lifeskim:mentions | umls-concept:C0025500 | lld:lifeskim |
| pubmed-article:2541616 | lifeskim:mentions | umls-concept:C0225335 | lld:lifeskim |
| pubmed-article:2541616 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:2541616 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:2541616 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2541616 | pubmed:dateCreated | 1989-6-20 | lld:pubmed |
| pubmed-article:2541616 | pubmed:abstractText | Normal human mesothelial (NHM) cells were transfected with a plasmid containing SV40 early region DNA. Individual colonies of transformed cells from several donors were subcultured for periods of 5 to 6 months and 60 to 70 population doublings (PDs) before senescence, in contrast to a culture lifespan of approximately 1 month and 15 PDs for NHM cells. One such culture, designated MeT-5A, escaped senescence and has been passaged continuously for more than 2 years. These cells had a single integrated copy of SV40 early region DNA in their genome, expressed SV40 large T antigen, and exhibited features of mesothelial cells including sensitivity to the cytotoxic effects of asbestos fibers. One year after injection subcutaneously or intraperitoneally in athymic nude mice, these cells remain nontumorigenic, and therefore are a potential model system for in vitro fiber carcinogenesis studies. | lld:pubmed |
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| pubmed-article:2541616 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2541616 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:2541616 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2541616 | pubmed:month | May | lld:pubmed |
| pubmed-article:2541616 | pubmed:issn | 0002-9440 | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:GerwinB IBI | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:HarrisC CCC | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:McMenaminM... | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:LechnerJ FJF | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:StahelR ARA | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:ReddelR RRR | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:JuHH | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:SomersA NAN | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:LaVeckM AMA | lld:pubmed |
| pubmed-article:2541616 | pubmed:author | pubmed-author:ReddelH KHK | lld:pubmed |
| pubmed-article:2541616 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2541616 | pubmed:volume | 134 | lld:pubmed |
| pubmed-article:2541616 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2541616 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2541616 | pubmed:pagination | 979-91 | lld:pubmed |
| pubmed-article:2541616 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2541616 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2541616 | pubmed:articleTitle | Establishment of a human in vitro mesothelial cell model system for investigating mechanisms of asbestos-induced mesothelioma. | lld:pubmed |
| pubmed-article:2541616 | pubmed:affiliation | Division of Cancer Etiology, National Cancer Institute, Bethesda, Maryland 20892. | lld:pubmed |
| pubmed-article:2541616 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2541616 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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