| pubmed-article:2460430 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C0022567 | lld:lifeskim |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C0221920 | lld:lifeskim |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C0007586 | lld:lifeskim |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C2825032 | lld:lifeskim |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:2460430 | lifeskim:mentions | umls-concept:C0450442 | lld:lifeskim |
| pubmed-article:2460430 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2460430 | pubmed:dateCreated | 1988-12-14 | lld:pubmed |
| pubmed-article:2460430 | pubmed:abstractText | Epidermal keratinocytes grow in culture to form a stratified squamous epithelium. These cultures contain a replicating as well as a terminally differentiating population and undergo surface desquamation. Epidermal growth factor (EGF) and cholera toxin are usually employed as growth-promoting agents because they reduce the population doubling time; that is, the period required to increase the total cell number twofold. There are three ways in which this reduction in population doubling time could be achieved: (a) the time for one cell cycle or the cell cycle length may be shortened; (b) the number of cells that withdraw from the cell cycle and terminally differentiate may be reduced; or (c) the number of cells that desquamate into the medium over a set period of time may be reduced. We have explored these possibilities in growing cultures of epidermal keratinocytes using a newly developed double-label assay. This assay gives a measure of both cell length and cell cycle withdrawal. Results show that the growth enhancement induced by EGF and cholera toxin can be attributed primarily to a reduction in cell cycle withdrawal and, to a lesser degree, to a reduction in cell cycle length. EGF and cholera toxin have no significant effect on the rate of desquamation. A linear correlation was noted between cell cycle lengths and withdrawal, suggesting an interconnection between the rate of cell renewal and the likelihood of undergoing terminal differentiation. | lld:pubmed |
| pubmed-article:2460430 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2460430 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2460430 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2460430 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2460430 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2460430 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2460430 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2460430 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2460430 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2460430 | pubmed:issn | 0883-8364 | lld:pubmed |
| pubmed-article:2460430 | pubmed:author | pubmed-author:SoroffH SHS | lld:pubmed |
| pubmed-article:2460430 | pubmed:author | pubmed-author:TaichmanL BLB | lld:pubmed |
| pubmed-article:2460430 | pubmed:author | pubmed-author:SetzerR WRW | lld:pubmed |
| pubmed-article:2460430 | pubmed:author | pubmed-author:GreifFF | lld:pubmed |
| pubmed-article:2460430 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2460430 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:2460430 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2460430 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2460430 | pubmed:pagination | 985-9 | lld:pubmed |
| pubmed-article:2460430 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:meshHeading | pubmed-meshheading:2460430-... | lld:pubmed |
| pubmed-article:2460430 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2460430 | pubmed:articleTitle | The effect of growth-promoting agents on replication and cell cycle withdrawal in cultures of epidermal keratinocytes. | lld:pubmed |
| pubmed-article:2460430 | pubmed:affiliation | Department of Surgery, School of Medicine, State University of New York, Stony Brook. | lld:pubmed |
| pubmed-article:2460430 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2460430 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2460430 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
