pubmed-article:2455217 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C0003316 | lld:lifeskim |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C0001492 | lld:lifeskim |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C1883712 | lld:lifeskim |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
pubmed-article:2455217 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
pubmed-article:2455217 | pubmed:issue | 5 | lld:pubmed |
pubmed-article:2455217 | pubmed:dateCreated | 1988-7-29 | lld:pubmed |
pubmed-article:2455217 | pubmed:abstractText | We developed a method for immunoaffinity purification of Saccharomyces cerevisiae adenylyl cyclase based on creating a fusion with a small peptide epitope. Using oligonucleotide technology to encode the peptide epitope we constructed a plasmid that expressed the fusion protein from the S. cerevisiae alcohol dehydrogenase promoter ADH1. A monoclonal antibody previously raised against the peptide was used to purify adenylyl cyclase by affinity chromatography. The purified enzyme appeared to be a multisubunit complex consisting of the 200-kilodalton adenylyl cyclase fusion protein and an unidentified 70-kilodalton protein. The purified protein could be activated by RAS proteins. Activation had an absolute requirement for a guanine nucleoside triphosphate. | lld:pubmed |
pubmed-article:2455217 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:language | eng | lld:pubmed |
pubmed-article:2455217 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:2455217 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:2455217 | pubmed:month | May | lld:pubmed |
pubmed-article:2455217 | pubmed:issn | 0270-7306 | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:WilsonI AIA | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:NikawaJJ | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:LernerR ARA | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:WiglerMM | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:FieldJJ | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:MacDonaldBB | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:RodgersLL | lld:pubmed |
pubmed-article:2455217 | pubmed:author | pubmed-author:BroekDD | lld:pubmed |
pubmed-article:2455217 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:2455217 | pubmed:volume | 8 | lld:pubmed |
pubmed-article:2455217 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:2455217 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:2455217 | pubmed:pagination | 2159-65 | lld:pubmed |
pubmed-article:2455217 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:meshHeading | pubmed-meshheading:2455217-... | lld:pubmed |
pubmed-article:2455217 | pubmed:year | 1988 | lld:pubmed |
pubmed-article:2455217 | pubmed:articleTitle | Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. | lld:pubmed |
pubmed-article:2455217 | pubmed:affiliation | Cold Spring Harbor Laboratory, New York 11724. | lld:pubmed |
pubmed-article:2455217 | pubmed:publicationType | Journal Article | lld:pubmed |