| pubmed-article:2426338 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C0008109 | lld:lifeskim |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C0301872 | lld:lifeskim |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C0487602 | lld:lifeskim |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C0205173 | lld:lifeskim |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:2426338 | lifeskim:mentions | umls-concept:C0444498 | lld:lifeskim |
| pubmed-article:2426338 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2426338 | pubmed:dateCreated | 1986-9-17 | lld:pubmed |
| pubmed-article:2426338 | pubmed:abstractText | After incubation of tissue sections with anti-allotype-enzyme conjugates, the localization of immunoglobulin-allotype-bearing cells in the lymphoid tissues of conventional and chimeric rabbits could be established. The use of anti-allotype sera bearing distinct enzyme labels allowed simultaneous recognition of B cells producing immunoglobulin of one or the other parental types in heterozygous rabbits, or of B cells from the donor and recipient in chimeras. After immunization of chimeric rabbits with trinitrophenyl-keyhole limpet hemocyanin, anti-trinitrophenyl antibody-forming cells could be demonstrated through the use of a trinitrophenyl-alkaline phosphatase conjugate. Simultaneous incubation of sections with this reagent and with horseradish peroxidase coupled to (donor or recipient) anti-allotype sera made possible the determination of the origin (donor or recipient) of the antibody-forming cells. In agreement with the results of plaque assays and analyses of serum antibodies, all the anti-TNP producing cells were of donor origin when the chimeras had been created through injection of spleen or lymph node cells from trinitrophenyl primed donors. With this study we introduce a simple, direct method for the simultaneous identification of cells that produce antibody of a given allotype and a given specificity, applicable to appropriate studies in heterozygous or chimeric rabbits. The procedure has various advantages over previously reported methods. | lld:pubmed |
| pubmed-article:2426338 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2426338 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2426338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2426338 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2426338 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2426338 | pubmed:issn | 0022-1554 | lld:pubmed |
| pubmed-article:2426338 | pubmed:author | pubmed-author:AdlerL TLT | lld:pubmed |
| pubmed-article:2426338 | pubmed:author | pubmed-author:AdlerF LFL | lld:pubmed |
| pubmed-article:2426338 | pubmed:author | pubmed-author:ClaassenEE | lld:pubmed |
| pubmed-article:2426338 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2426338 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:2426338 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2426338 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2426338 | pubmed:pagination | 989-94 | lld:pubmed |
| pubmed-article:2426338 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:meshHeading | pubmed-meshheading:2426338-... | lld:pubmed |
| pubmed-article:2426338 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:2426338 | pubmed:articleTitle | Double immunocytochemical staining for the in situ study of allotype distribution during an anti-trinitrophenyl immune response in chimeric rabbits. | lld:pubmed |
| pubmed-article:2426338 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2426338 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2426338 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2426338 | lld:pubmed |
