| pubmed-article:2403525 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0935640 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0021585 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0079460 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C1333104 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C1705099 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2403525 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2403525 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2403525 | pubmed:dateCreated | 1990-2-8 | lld:pubmed |
| pubmed-article:2403525 | pubmed:abstractText | A plasmid pAc373GM-CSF was constructed and co-transfected into Spodoptera frugiperda (Sf9) cells with wild-type Autographa californica nuclear polyhedrosis virus (AcNPV) DNA. The recombinant virus vAc373GM-CSF was identified and purified by several rounds of plaque hybridization. By assaying the culture medium, we demonstrated recombinant virus infected Sf9 cells expressing hGM-CSF. Recombinant hGM-CSFs with apparent molecular masses of 14.5, 15.5 and 16.5 kDa were detected by the Western blot method. All 3 forms have biological activity of hGM-CSF. Following N-glycanase treatment, a single band of 14.5-15.5 kDa appeared in SDS-PAGE. Western blot analysis of expression in Sf9 cell treated with tunicamycin revealed only the presence of the 14.5 kDa species. Thus, the signal sequence of recombinant hGM-CSF could be recognized and cleaved by infected insect cell and the resultant molecule secreted into the media. | lld:pubmed |
| pubmed-article:2403525 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2403525 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2403525 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2403525 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2403525 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2403525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2403525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2403525 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2403525 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2403525 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:2403525 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:2403525 | pubmed:author | pubmed-author:WuM CMC | lld:pubmed |
| pubmed-article:2403525 | pubmed:author | pubmed-author:ChiouC JCJ | lld:pubmed |
| pubmed-article:2403525 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2403525 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2403525 | pubmed:volume | 259 | lld:pubmed |
| pubmed-article:2403525 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2403525 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2403525 | pubmed:pagination | 249-53 | lld:pubmed |
| pubmed-article:2403525 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:meshHeading | pubmed-meshheading:2403525-... | lld:pubmed |
| pubmed-article:2403525 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2403525 | pubmed:articleTitle | Expression of human granulocyte-macrophage colony-stimulating factor gene in insect cells by a baculovirus vector. | lld:pubmed |
| pubmed-article:2403525 | pubmed:affiliation | Department of Biochemistry, University of North Texas/Texas College of Osteopathic Medicine, Denton 76203. | lld:pubmed |
| pubmed-article:2403525 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2403525 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2403525 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2403525 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2403525 | lld:pubmed |
