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pubmed-article:2268281pubmed:abstractTextSelenium (Se) deficiency decreased by 8-fold the activity of type 1 iodothyronine 5'-deiodinase (ID-I) in hepatic microsomal fractions from rats. Solubilized hepatic microsomes from rats injected with 75Se-labelled Na2SeO3 4 days before killing were found by chromatography on agarose gels to contain a 75Se-containing fraction with ID-I activity. PAGE of this fraction under reducing conditions, followed by autoradiography, revealed a single 75Se-containing protein (Mr 27,400 +/- 300). This protein could also be labelled with 125I-bromoacetyl reverse tri-iodothyronine, an affinity label for ID-I. The results suggest that hepatic ID-I is a selenoprotein or has an Se-containing subunit essential for activity.lld:pubmed
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pubmed-article:2268281pubmed:articleTitleHepatic iodothyronine 5'-deiodinase. The role of selenium.lld:pubmed
pubmed-article:2268281pubmed:affiliationDivision of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen, U.K.lld:pubmed
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