| pubmed-article:2237639 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2237639 | lifeskim:mentions | umls-concept:C0085080 | lld:lifeskim |
| pubmed-article:2237639 | lifeskim:mentions | umls-concept:C1708726 | lld:lifeskim |
| pubmed-article:2237639 | lifeskim:mentions | umls-concept:C1366623 | lld:lifeskim |
| pubmed-article:2237639 | lifeskim:mentions | umls-concept:C0872178 | lld:lifeskim |
| pubmed-article:2237639 | lifeskim:mentions | umls-concept:C0205227 | lld:lifeskim |
| pubmed-article:2237639 | lifeskim:mentions | umls-concept:C1332838 | lld:lifeskim |
| pubmed-article:2237639 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2237639 | pubmed:dateCreated | 1990-12-10 | lld:pubmed |
| pubmed-article:2237639 | pubmed:abstractText | We demonstrate the feasibility of targeted gene replacement at an endogenous, chromosomal gene locus in cultured mammalian cells, employing a two-step strategy similar to an approach routinely used for genetic manipulation in yeast. Utilizing an APRT+ recombinant generated by targeted integration of plasmid sequences (including a functional copy of the gpt gene) at the CHO APRT locus, we have been able to select gpt- "pop-out" recombinants that have arisen by intrachromosomal recombination between APRT direct repeats at the targeted integration site. Reciprocal exchanges leading to "pop-out" of integrated plasmid/gpt gene sequences occur at a rate of approximately 6.3 x 10(-6) per cell generation. Depending on the site of crossover, such "pop-out" events result in either replacement or restoration of the original APRT target gene sequence. | lld:pubmed |
| pubmed-article:2237639 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2237639 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2237639 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2237639 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2237639 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2237639 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2237639 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2237639 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2237639 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2237639 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2237639 | pubmed:issn | 0740-7750 | lld:pubmed |
| pubmed-article:2237639 | pubmed:author | pubmed-author:NairnR SRS | lld:pubmed |
| pubmed-article:2237639 | pubmed:author | pubmed-author:AdairG MGM | lld:pubmed |
| pubmed-article:2237639 | pubmed:author | pubmed-author:WilsonJ HJH | lld:pubmed |
| pubmed-article:2237639 | pubmed:author | pubmed-author:ScheererJ BJB | lld:pubmed |
| pubmed-article:2237639 | pubmed:author | pubmed-author:BrothermanK... | lld:pubmed |
| pubmed-article:2237639 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2237639 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:2237639 | pubmed:geneSymbol | gpt | lld:pubmed |
| pubmed-article:2237639 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2237639 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2237639 | pubmed:pagination | 437-41 | lld:pubmed |
| pubmed-article:2237639 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2237639 | pubmed:meshHeading | pubmed-meshheading:2237639-... | lld:pubmed |
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| pubmed-article:2237639 | pubmed:meshHeading | pubmed-meshheading:2237639-... | lld:pubmed |
| pubmed-article:2237639 | pubmed:meshHeading | pubmed-meshheading:2237639-... | lld:pubmed |
| pubmed-article:2237639 | pubmed:meshHeading | pubmed-meshheading:2237639-... | lld:pubmed |
| pubmed-article:2237639 | pubmed:meshHeading | pubmed-meshheading:2237639-... | lld:pubmed |
| pubmed-article:2237639 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2237639 | pubmed:articleTitle | Targeted gene replacement at the endogenous APRT locus in CHO cells. | lld:pubmed |
| pubmed-article:2237639 | pubmed:affiliation | University of Texas M.D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957. | lld:pubmed |
| pubmed-article:2237639 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2237639 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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