| pubmed-article:2200305 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2200305 | lifeskim:mentions | umls-concept:C0014444 | lld:lifeskim |
| pubmed-article:2200305 | lifeskim:mentions | umls-concept:C1959616 | lld:lifeskim |
| pubmed-article:2200305 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:2200305 | lifeskim:mentions | umls-concept:C0806140 | lld:lifeskim |
| pubmed-article:2200305 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:2200305 | lifeskim:mentions | umls-concept:C1708513 | lld:lifeskim |
| pubmed-article:2200305 | lifeskim:mentions | umls-concept:C0598014 | lld:lifeskim |
| pubmed-article:2200305 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2200305 | pubmed:dateCreated | 1990-9-13 | lld:pubmed |
| pubmed-article:2200305 | pubmed:abstractText | Two alternative procedures are described for the quantitative determination of phosphatidylcholine in a flow-injection system utilizing immobilized enzymes. Phospholipase C from Bacillus cereus and phospholipase D from cabbage were covalently bound to the surface of controlled-pore glass beads and the enzyme-derivatized beads were packed in small columns. In the first procedure, the phospholipase C column was connected with a second column containing coimmobilized alkaline phosphatase and choline oxidase. In the alternative procedure, the column packed with immobilized phospholipase D was connected with a column packed with immobilized choline oxidase. The hydrogen peroxide produced through the action of choline oxidase in both flow-injection systems was detected amperometrically. Both procedures are suitable for an accurate and rapid quantitation of phosphatidylcholine. The sensitivity of the method based on phospholipase C and alkaline phosphatase is higher than that using phospholipase D. Quantitation of phosphatidylcholine at the nanomole level can be easily obtained using the first method. | lld:pubmed |
| pubmed-article:2200305 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2200305 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2200305 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2200305 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2200305 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2200305 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2200305 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2200305 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2200305 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2200305 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2200305 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:2200305 | pubmed:author | pubmed-author:RobertsDD | lld:pubmed |
| pubmed-article:2200305 | pubmed:author | pubmed-author:BinagliaLL | lld:pubmed |
| pubmed-article:2200305 | pubmed:author | pubmed-author:MasoomMM | lld:pubmed |
| pubmed-article:2200305 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2200305 | pubmed:volume | 187 | lld:pubmed |
| pubmed-article:2200305 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2200305 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2200305 | pubmed:pagination | 240-5 | lld:pubmed |
| pubmed-article:2200305 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:meshHeading | pubmed-meshheading:2200305-... | lld:pubmed |
| pubmed-article:2200305 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2200305 | pubmed:articleTitle | Determination of phosphatidylcholine in a flow injection system using immobilized enzyme reactors. | lld:pubmed |
| pubmed-article:2200305 | pubmed:affiliation | Institute of Biochemistry, University of Baluchistan, Quetta, Pakistan. | lld:pubmed |
| pubmed-article:2200305 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2200305 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2200305 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2200305 | lld:pubmed |
