| pubmed-article:2194399 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2194399 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:2194399 | lifeskim:mentions | umls-concept:C0162772 | lld:lifeskim |
| pubmed-article:2194399 | lifeskim:mentions | umls-concept:C0162524 | lld:lifeskim |
| pubmed-article:2194399 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:2194399 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:2194399 | lifeskim:mentions | umls-concept:C0126266 | lld:lifeskim |
| pubmed-article:2194399 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2194399 | pubmed:dateCreated | 1990-8-8 | lld:pubmed |
| pubmed-article:2194399 | pubmed:abstractText | Addition of oxygen to whole cells of Escherichia coli suspended in the presence of the chemiluminescent probe bis-N-methylacridinium nitrate (lucigenin) resulted in a light emission increase of 200% of control. Addition of air to cells showed a chemiluminescent response far less than the response to oxygen. The redox cycling agents paraquat and menadione, which are known to increase intracellular production of O2- and H2O2, were also found to cause a measurable increase in lucigenin chemiluminescence in E. coli cells when added at concentrations of 1 and 0.1 mM, respectively. The oxygen-induced chemiluminescent response was not suppressed by extracellularly added superoxide dismutase or catalase. Further, the lucigenin-dependent chemiluminescent response of aerobically grown E. coli to oxygen was significantly greater than that of cells grown anaerobically. Heat-killed cells showed no increase in chemiluminescence on the addition of either oxygen, paraquat, or menadione. These results show that lucigenin may be used as a chemiluminescent probe to demonstrate continuous intracellular production of reactive oxygen metabolites in E. coli. | lld:pubmed |
| pubmed-article:2194399 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2194399 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2194399 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2194399 | pubmed:month | May | lld:pubmed |
| pubmed-article:2194399 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:2194399 | pubmed:author | pubmed-author:SENAAA | lld:pubmed |
| pubmed-article:2194399 | pubmed:author | pubmed-author:GoulbourneE... | lld:pubmed |
| pubmed-article:2194399 | pubmed:author | pubmed-author:PetersT RTR | lld:pubmed |
| pubmed-article:2194399 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2194399 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2194399 | pubmed:volume | 186 | lld:pubmed |
| pubmed-article:2194399 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2194399 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2194399 | pubmed:pagination | 316-9 | lld:pubmed |
| pubmed-article:2194399 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:meshHeading | pubmed-meshheading:2194399-... | lld:pubmed |
| pubmed-article:2194399 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2194399 | pubmed:articleTitle | Lucigenin chemiluminescence as a probe for measuring reactive oxygen species production in Escherichia coli. | lld:pubmed |
| pubmed-article:2194399 | pubmed:affiliation | Department of Microbiology, Loma Linda University, California 92350. | lld:pubmed |
| pubmed-article:2194399 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2194399 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2194399 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2194399 | lld:pubmed |
