| pubmed-article:2185112 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2185112 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:2185112 | lifeskim:mentions | umls-concept:C0063684 | lld:lifeskim |
| pubmed-article:2185112 | lifeskim:mentions | umls-concept:C1415831 | lld:lifeskim |
| pubmed-article:2185112 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:2185112 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:2185112 | pubmed:dateCreated | 1990-6-5 | lld:pubmed |
| pubmed-article:2185112 | pubmed:abstractText | Islet amyloid polypeptide (IAPP) has been identified as the major constituent of the pancreatic amyloid of non-insulin-dependent diabetes mellitus (NIDDM) and is also present in normal beta-cell secretory granules. To determine whether IAPP is a pancreatic secretory product, we measured the quantity of IAPP-like immunoreactivity (IAPP-LI), insulin, and glucagon released into 5 ml of incubation medium during a 2-h incubation of monolayer cultures (n = 5) of neonatal (3- to 5-day-old) Sprague-Dawley rat pancreases under three conditions: 1.67 mM glucose, 16.7 mM glucose, and 16.7 mM glucose plus 10 mM arginine and 0.1 mM isobutylmethylxanthine (IBMX). The quantity of IAPP-LI, insulin, and glucagon in the cell extract was also determined. Mean +/- SE IAPP-LI in the incubation medium increased from 0.041 +/- 0.003 pmol in 1.67 mM glucose to 0.168 +/- 0.029 pmol in 16.7 mM glucose (P less than 0.05) and 1.02 +/- 0.06 pmol in 16.7 mM glucose plus arginine and IBMX (P less than 0.05 vs. 1.67 or 16.7 mM glucose). Insulin secretion increased similarly from 4.34 +/- 0.27 to 20.2 +/- 0.6 pmol (P less than 0.05) and then to 135 +/- 5 pmol (P less than 0.05 vs. 1.67 or 16.7 mM glucose). Glucagon release tended to decrease with the increase in glucose concentration (0.39 +/- 0.01 vs. 0.33 +/- 0.02 pmol, P less than 0.1), whereas with the addition of arginine and IBMX to high glucose, glucagon release increased to 1.32 +/- 0.03 pmol (P less than 0.05 vs. 1.67 or 16.7 mM glucose).(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
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| pubmed-article:2185112 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2185112 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2185112 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2185112 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2185112 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2185112 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2185112 | pubmed:month | May | lld:pubmed |
| pubmed-article:2185112 | pubmed:issn | 0012-1797 | lld:pubmed |
| pubmed-article:2185112 | pubmed:author | pubmed-author:KahnS ESE | lld:pubmed |
| pubmed-article:2185112 | pubmed:author | pubmed-author:PorteDDJr | lld:pubmed |
| pubmed-article:2185112 | pubmed:author | pubmed-author:FujimotoW YWY | lld:pubmed |
| pubmed-article:2185112 | pubmed:author | pubmed-author:EnsinckJ WJW | lld:pubmed |
| pubmed-article:2185112 | pubmed:author | pubmed-author:TaborskyG... | lld:pubmed |
| pubmed-article:2185112 | pubmed:author | pubmed-author:SchwartzM WMW | lld:pubmed |
| pubmed-article:2185112 | pubmed:author | pubmed-author:D'AlessioD... | lld:pubmed |
| pubmed-article:2185112 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2185112 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:2185112 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2185112 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2185112 | pubmed:pagination | 634-8 | lld:pubmed |
| pubmed-article:2185112 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:2185112 | pubmed:meshHeading | pubmed-meshheading:2185112-... | lld:pubmed |
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| pubmed-article:2185112 | pubmed:meshHeading | pubmed-meshheading:2185112-... | lld:pubmed |
| pubmed-article:2185112 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2185112 | pubmed:articleTitle | Evidence of cosecretion of islet amyloid polypeptide and insulin by beta-cells. | lld:pubmed |
| pubmed-article:2185112 | pubmed:affiliation | Department of Medicine, University of Washington, Seattle. | lld:pubmed |
| pubmed-article:2185112 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2185112 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2185112 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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