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21586880

Source:http://linkedlifedata.com/resource/pubmed/id/21586880

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pubmed-article:21586880pubmed:dateCreated2011-5-18lld:pubmed
pubmed-article:21586880pubmed:abstractTextA simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be about 2.5 × 10(3) cfu/mL and was similar to that of the standard PCR. We compared the specificity and sensitivity of the simplified PCR to those of a culture method. Out of 1,685 milk samples cultured in mycoplasma broth, the simplified PCR detected Mycoplasma DNA in 152 that were also positive according to the culture assay. The sensitivity and specificity of the simplified PCR were 98.7% and 99.7%, respectively, for detecting mycoplasma in those cultures. The results obtained by the simplified PCR were consistent with ones from standard PCR. This newly developed simplified PCR, which does not require DNA purification, can analyze about 300 cultured samples within 3 h. The results from our study suggest that the simplified PCR can be used for mycoplasma mastitis screening in large-scale dairy farms.lld:pubmed
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pubmed-article:21586880pubmed:authorpubmed-author:YokotaHiroshi...lld:pubmed
pubmed-article:21586880pubmed:authorpubmed-author:OhtaTakehiroTlld:pubmed
pubmed-article:21586880pubmed:authorpubmed-author:TamuraYutakaYlld:pubmed
pubmed-article:21586880pubmed:authorpubmed-author:KawaiKazuhiro...lld:pubmed
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pubmed-article:21586880pubmed:authorpubmed-author:ItoNobuhikoNlld:pubmed
pubmed-article:21586880pubmed:authorpubmed-author:HiguchiHideto...lld:pubmed
pubmed-article:21586880pubmed:authorpubmed-author:IwanoHidetomo...lld:pubmed
pubmed-article:21586880pubmed:authorpubmed-author:ObayashiTetsu...lld:pubmed
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pubmed-article:21586880pubmed:year2011lld:pubmed
pubmed-article:21586880pubmed:articleTitleA simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle.lld:pubmed
pubmed-article:21586880pubmed:affiliationAnimal Health Laboratory, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido 069-8501, Japan. higuchi@rakuno.ac.jplld:pubmed
pubmed-article:21586880pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21586880pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:21586880pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed