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pubmed-article:21402102pubmed:abstractTextIsolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres.lld:pubmed
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pubmed-article:21402102pubmed:authorpubmed-author:PurowBenjamin...lld:pubmed
pubmed-article:21402102pubmed:authorpubmed-author:ShermanJonath...lld:pubmed
pubmed-article:21402102pubmed:authorpubmed-author:RedickJan AJAlld:pubmed
pubmed-article:21402102pubmed:copyrightInfoCopyright © 2011 Elsevier B.V. All rights reserved.lld:pubmed
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pubmed-article:21402102pubmed:articleTitleA novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells.lld:pubmed
pubmed-article:21402102pubmed:affiliationDepartment of Neurological Surgery, University of Virginia, Charlottesville, Virginia, USA. jsherman0620@gmail.comlld:pubmed
pubmed-article:21402102pubmed:publicationTypeJournal Articlelld:pubmed