| pubmed-article:2137121 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C0031299 | lld:lifeskim |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C0040329 | lld:lifeskim |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C0315225 | lld:lifeskim |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C0314603 | lld:lifeskim |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C1136169 | lld:lifeskim |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:2137121 | lifeskim:mentions | umls-concept:C0336791 | lld:lifeskim |
| pubmed-article:2137121 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2137121 | pubmed:dateCreated | 1990-3-14 | lld:pubmed |
| pubmed-article:2137121 | pubmed:abstractText | Erwinia amylovora 1430 was shown to be sensitive to Mu G(-) particles. Infection resulted either in lytic development or in lysogenic derivatives with insertion of the Mu genome at many sites in the bacterial chromosome. We used the Mu d1Bx::Tn9 (lac Apr Cmr) derivative, called Mu dX, to identify mutants affected in pathogenicity and in their ability to induce a hypersensitive reaction (HR) on tobacco plants. Inoculation of 1,400 lysogenic derivatives on apple root calli led to the identification of 12 mutants in three classes: (i) class 1 mutants were nonpathogenic and unable to induce an HR on tobacco plants; (ii) class 2 mutants were nonpathogenic but retained the ability to induce an HR; and (iii) class 3 mutants showed attenuated virulence. Of the 12 mutants, 8 had a single insertion of the Mu dX prophage. For class 1 and 2 mutants, reversion to pathogenicity was concomitant with the loss of the Mu dX prophage. Furthermore, revertants from the class 1 mutants also recovered the ability to induce an HR on tobacco plants. Five of the six class 3 mutants were impaired in exopolysaccharide production. No changes of the envelope structure (lipopolysaccharide and outer membrane proteins) were correlated with differences in pathogenicity. One class 3 mutant did not produce any functional siderophore, suggesting that iron uptake could be involved in pathogenicity. | lld:pubmed |
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| pubmed-article:2137121 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2137121 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2137121 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2137121 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:2137121 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:2137121 | pubmed:author | pubmed-author:VannesteJ LJL | lld:pubmed |
| pubmed-article:2137121 | pubmed:author | pubmed-author:ExpertDD | lld:pubmed |
| pubmed-article:2137121 | pubmed:author | pubmed-author:PaulinJ PJP | lld:pubmed |
| pubmed-article:2137121 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2137121 | pubmed:volume | 172 | lld:pubmed |
| pubmed-article:2137121 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2137121 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2137121 | pubmed:pagination | 932-41 | lld:pubmed |
| pubmed-article:2137121 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
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| pubmed-article:2137121 | pubmed:meshHeading | pubmed-meshheading:2137121-... | lld:pubmed |
| pubmed-article:2137121 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2137121 | pubmed:articleTitle | Bacteriophage Mu as a genetic tool to study Erwinia amylovora pathogenicity and hypersensitive reaction on tobacco. | lld:pubmed |
| pubmed-article:2137121 | pubmed:affiliation | Pathologie Végétale, Paris, France. | lld:pubmed |
| pubmed-article:2137121 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2137121 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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