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pubmed-article:21260998pubmed:dateCreated2011-1-24lld:pubmed
pubmed-article:21260998pubmed:abstractTextWhether the MDCK cell line might adapt to grow in serum-free Hybris-2 medium and influenza viruses might be reproduced in the adapted cells was studied. Seventeen passages using the Hybris-2 medium yielded cells adapted to growth in this medium (MDCK-BS). The reproduction of influenza A (H1N1 and H3N2) and B viruses versus the cells cultured in Eagle's medium was studied. The laboratory strain of influenza A/Aichi/1/68 (H3N2) and the strain B/Ohio/01/05 of influenza B in equal titers were shown to be reproduced in both control cells on Eagle's medium and MDCK-BS cells adapted to growth in the Hybris-2 medium. The reproduction of the strains A/Brisbane/10/07 (H3N2) and A/Solomon Islands/3/06 (H1N1) was less active in the MDCK cells. Each strain of influenza viruses displayed varying infective activities. The developed serum-free Hybris-2 medium may be used for cultivation of monolayer continuous MDCK cells and for their reproduction of influenza A and B viruses.lld:pubmed
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pubmed-article:21260998pubmed:authorpubmed-author:IsaevaE IEIlld:pubmed
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pubmed-article:21260998pubmed:volume55lld:pubmed
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pubmed-article:21260998pubmed:articleTitle[Influenza virus reproduction in the MDCK cells adapted to growth in serum-free Hybris-2 medium].lld:pubmed
pubmed-article:21260998pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:21260998pubmed:publicationTypeEnglish Abstractlld:pubmed