| pubmed-article:21248753 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21248753 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
| pubmed-article:21248753 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:21248753 | lifeskim:mentions | umls-concept:C1428449 | lld:lifeskim |
| pubmed-article:21248753 | lifeskim:mentions | umls-concept:C1709059 | lld:lifeskim |
| pubmed-article:21248753 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:21248753 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:21248753 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:21248753 | pubmed:dateCreated | 2011-2-8 | lld:pubmed |
| pubmed-article:21248753 | pubmed:abstractText | The ability to direct functional proteins to specific DNA sequences is a long-sought goal in the study and engineering of biological processes. Transcription activator-like effectors (TALEs) from Xanthomonas sp. are site-specific DNA-binding proteins that can be readily designed to target new sequences. Because TALEs contain a large number of repeat domains, it can be difficult to synthesize new variants. Here we describe a method that overcomes this problem. We leverage codon degeneracy and type IIs restriction enzymes to generate orthogonal ligation linkers between individual repeat monomers, thus allowing full-length, customized, repeat domains to be constructed by hierarchical ligation. We synthesized 17 TALEs that are customized to recognize specific DNA-binding sites, and demonstrate that they can specifically modulate transcription of endogenous genes (SOX2 and KLF4) in human cells. | lld:pubmed |
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| pubmed-article:21248753 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21248753 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21248753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21248753 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21248753 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:21248753 | pubmed:issn | 1546-1696 | lld:pubmed |
| pubmed-article:21248753 | pubmed:author | pubmed-author:ZhangFengF | lld:pubmed |
| pubmed-article:21248753 | pubmed:author | pubmed-author:ChurchGeorge... | lld:pubmed |
| pubmed-article:21248753 | pubmed:author | pubmed-author:ArlottaPaolaP | lld:pubmed |
| pubmed-article:21248753 | pubmed:author | pubmed-author:KosuriSriramS | lld:pubmed |
| pubmed-article:21248753 | pubmed:author | pubmed-author:CongLeL | lld:pubmed |
| pubmed-article:21248753 | pubmed:author | pubmed-author:LodatoSimonaS | lld:pubmed |
| pubmed-article:21248753 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21248753 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:21248753 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21248753 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21248753 | pubmed:pagination | 149-53 | lld:pubmed |
| pubmed-article:21248753 | pubmed:dateRevised | 2011-9-20 | lld:pubmed |
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| pubmed-article:21248753 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:21248753 | pubmed:articleTitle | Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. | lld:pubmed |
| pubmed-article:21248753 | pubmed:affiliation | Harvard University, Cambridge, Massachusetts, USA. | lld:pubmed |
| pubmed-article:21248753 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21248753 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:21248753 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:21248753 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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