| pubmed-article:21132961 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21132961 | lifeskim:mentions | umls-concept:C0376624 | lld:lifeskim |
| pubmed-article:21132961 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:21132961 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:21132961 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:21132961 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:21132961 | pubmed:dateCreated | 2010-12-7 | lld:pubmed |
| pubmed-article:21132961 | pubmed:abstractText | Currently, several beef processors employ test-and-hold systems for increased quality control of ground beef. In such programs, each lot of product must be tested and found negative for Escherichia coli O157:H7 prior to release of the product into commerce. Optimization of three testing attributes (detection time, specificity, and sensitivity) is critical to the success of such strategies. Because ground beef is a highly perishable product, the testing methodology used must be as rapid as possible. The test also must have a low false-positive result rate so product is not needlessly discarded. False-negative results cannot be tolerated because they would allow contaminated product to be released and potentially cause disease. In this study, two culture-based and three PCR-based methods for detecting E. coli O157:H7 in ground beef were compared for their abilities to meet the above criteria. Ground beef samples were individually spiked with five genetically distinct strains of E. coli O157: H7 at concentrations of 17 and 1.7 CFU/65 g and then subjected to the various testing methodologies. There was no difference (P > 0.05) in the abilities of the PCR-based methods to detect E. coli O157:H7 inoculated in ground beef at 1.7 CFU/65 g. The culture-based systems detected more positive samples than did the PCR-based systems, but the detection times (21 to 48 h) were at least 9 h longer than those for the PCR-based methods (7.5 to 12 h). Ground beef samples were also spiked with potentially cross-reactive strains. The PCR-based systems that employed an immunomagnetic separation step prior to detection produced fewer false-positive results. | lld:pubmed |
| pubmed-article:21132961 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21132961 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21132961 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21132961 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21132961 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:21132961 | pubmed:issn | 0362-028X | lld:pubmed |
| pubmed-article:21132961 | pubmed:author | pubmed-author:KoohmaraieMoh... | lld:pubmed |
| pubmed-article:21132961 | pubmed:author | pubmed-author:ArthurTerranc... | lld:pubmed |
| pubmed-article:21132961 | pubmed:author | pubmed-author:NouXiangwuX | lld:pubmed |
| pubmed-article:21132961 | pubmed:author | pubmed-author:BosilevacJose... | lld:pubmed |
| pubmed-article:21132961 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:21132961 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:21132961 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21132961 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21132961 | pubmed:pagination | 1566-74 | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:meshHeading | pubmed-meshheading:21132961... | lld:pubmed |
| pubmed-article:21132961 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:21132961 | pubmed:articleTitle | Evaluation of culture- and PCR-based detection methods for Escherichia coli O157:H7 in inoculated ground beeft. | lld:pubmed |
| pubmed-article:21132961 | pubmed:affiliation | U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA. arthur@email.marc.usda.gov | lld:pubmed |
| pubmed-article:21132961 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21132961 | pubmed:publicationType | Comparative Study | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:21132961 | lld:pubmed |
