| pubmed-article:21067307 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:21067307 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:21067307 | lifeskim:mentions | umls-concept:C0063684 | lld:lifeskim |
| pubmed-article:21067307 | lifeskim:mentions | umls-concept:C0600688 | lld:lifeskim |
| pubmed-article:21067307 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:21067307 | lifeskim:mentions | umls-concept:C1415831 | lld:lifeskim |
| pubmed-article:21067307 | lifeskim:mentions | umls-concept:C0010467 | lld:lifeskim |
| pubmed-article:21067307 | pubmed:issue | 3-4 | lld:pubmed |
| pubmed-article:21067307 | pubmed:dateCreated | 2010-11-16 | lld:pubmed |
| pubmed-article:21067307 | pubmed:abstractText | Type 2 diabetes involves aberrant misfolding of human islet amyloid polypeptide (h-IAPP) and resultant pancreatic amyloid deposits. Curcumin, a biphenolic small molecule, has offered potential benefits in other protein misfolding diseases, such as Alzheimer's disease. Our aim was to investigate whether curcumin alters h-IAPP misfolding and protects from cellular toxicity at physiologically relevant concentrations. The effect of curcumin on h-IAPP misfolding in vitro was investigated by electron paramagnetic resonance spectroscopy, ThT fluorescence and electron microscopy. Our in vitro studies revealed that curcumin significantly reduces h-IAPP fibril formation and aggregates formed in the presence of curcumin display alternative morphology and structure. We then tested a potential protective effect of curcumin against h-IAPP toxicity on ?-cells. Micromolar concentrations of curcumin partially protect INS cells from exogenous IAPP toxicity. This protective effect, however, is limited to a narrow concentration range, as curcumin becomes cytotoxic at micromolar concentrations. In different models of endogenous over-expression of h-IAPP (INS cells and h-IAPP transgenic rat islets), curcumin failed to protect ?-cells from h-IAPP-induced apoptosis. While curcumin has the ability to inhibit amyloid formation, the present data suggest that, without further modification, it is unlikely to be therapeutically useful in protection of ?-cells in type 2 diabetes. | lld:pubmed |
| pubmed-article:21067307 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21067307 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21067307 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21067307 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21067307 | pubmed:language | eng | lld:pubmed |
| pubmed-article:21067307 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21067307 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:21067307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21067307 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:21067307 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:21067307 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:21067307 | pubmed:issn | 1744-2818 | lld:pubmed |
| pubmed-article:21067307 | pubmed:author | pubmed-author:LangenRalfR | lld:pubmed |
| pubmed-article:21067307 | pubmed:author | pubmed-author:ButlerPeter... | lld:pubmed |
| pubmed-article:21067307 | pubmed:author | pubmed-author:BedroodSaharS | lld:pubmed |
| pubmed-article:21067307 | pubmed:author | pubmed-author:GurloTatyanaT | lld:pubmed |
| pubmed-article:21067307 | pubmed:author | pubmed-author:CostesSafiaS | lld:pubmed |
| pubmed-article:21067307 | pubmed:author | pubmed-author:DavalMarieM | lld:pubmed |
| pubmed-article:21067307 | pubmed:author | pubmed-author:HuangChang-Ji... | lld:pubmed |
| pubmed-article:21067307 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:21067307 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:21067307 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:21067307 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:21067307 | pubmed:pagination | 118-28 | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:meshHeading | pubmed-meshheading:21067307... | lld:pubmed |
| pubmed-article:21067307 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:21067307 | pubmed:articleTitle | The effect of curcumin on human islet amyloid polypeptide misfolding and toxicity. | lld:pubmed |
| pubmed-article:21067307 | pubmed:affiliation | Larry Hillblom Islet Research Center, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA. | lld:pubmed |
| pubmed-article:21067307 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:21067307 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:21067307 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:21067307 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
