Linked Life Data

20960307

Source:http://linkedlifedata.com/resource/pubmed/id/20960307

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:20960307rdf:typepubmed:Citationlld:pubmed
pubmed-article:20960307lifeskim:mentionsumls-concept:C0206679lld:lifeskim
pubmed-article:20960307lifeskim:mentionsumls-concept:C0002062lld:lifeskim
pubmed-article:20960307lifeskim:mentionsumls-concept:C0146898lld:lifeskim
pubmed-article:20960307lifeskim:mentionsumls-concept:C0441655lld:lifeskim
pubmed-article:20960307lifeskim:mentionsumls-concept:C0439810lld:lifeskim
pubmed-article:20960307lifeskim:mentionsumls-concept:C1533691lld:lifeskim
pubmed-article:20960307pubmed:issue2lld:pubmed
pubmed-article:20960307pubmed:dateCreated2010-10-20lld:pubmed
pubmed-article:20960307pubmed:abstractTextHerpes simplex virus type 1 (HSV-1) is a commonly occurring human pathogen worldwide. There is an urgent need to discover and develop new alternative agents for the management of HSV-1 infection. Tripterygium hypoglaucum (level) Hutch (Celastraceae) is a traditional Chinese medicine plant with many pharmacological activities such as anti-inflammation, anti-tumor and antifertility. The usual medicinal part is the roots which contain about a 1% yield of alkaloids. A crude total alkaloids extract was prepared from the roots of T. hypoglaucum amd its antiviral activity against HSV-1 in Vero cells was evaluated by cytopathic effect (CPE) assay, plaque reduction assay and by RT-PCR analysis. The alkaloids extract presented low cytotoxicity (CC(50) = 46.6 ?g/mL) and potent CPE inhibition activity, the 50% inhibitory concentration (IC(50)) was 6.5 ?g/mL, noticeably lower than that of Acyclovir (15.4 ?g /mL). Plaque formation was significantly reduced by the alkaloids extract at concentrations of 6.25 ?g/mL to 12.5 ?g/mL, the plaque reduction ratio reached 55% to 75 which was 35% higher than that of Acyclovir at the same concentration. RT-PCR analysis showed that, the transcription of two important delayed early genes UL30 and UL39, and a late gene US6 of HSV-1 genome all were suppressed by the alkaloids extract, the expression inhibiting efficacy compared to the control was 74.6% (UL30), 70.9% (UL39) and 62.6% (US6) respectively at the working concentration of 12.5 ?g/mL. The above results suggest a potent anti-HSV-1 activity of the alkaloids extract in vitro.lld:pubmed
pubmed-article:20960307pubmed:languageenglld:pubmed
pubmed-article:20960307pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:20960307pubmed:citationSubsetIMlld:pubmed
pubmed-article:20960307pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:20960307pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:20960307pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:20960307pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:20960307pubmed:statusMEDLINElld:pubmed
pubmed-article:20960307pubmed:monthAprlld:pubmed
pubmed-article:20960307pubmed:issn1995-820Xlld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:ChuI SISlld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:WeiXiao-yiXYlld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:YangChong-ren...lld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:WangLian-junL...lld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:LuDa-xiangDXlld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:WangYi-feiYFlld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:ZhangChuan-ha...lld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:ZhangYing-jun...lld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:QiRen-binRBlld:pubmed
pubmed-article:20960307pubmed:authorpubmed-author:CuiYun-xiaYXlld:pubmed
pubmed-article:20960307pubmed:issnTypeElectroniclld:pubmed
pubmed-article:20960307pubmed:volume25lld:pubmed
pubmed-article:20960307pubmed:ownerNLMlld:pubmed
pubmed-article:20960307pubmed:authorsCompleteYlld:pubmed
pubmed-article:20960307pubmed:pagination107-14lld:pubmed
pubmed-article:20960307pubmed:dateRevised2011-11-17lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:meshHeadingpubmed-meshheading:20960307...lld:pubmed
pubmed-article:20960307pubmed:year2010lld:pubmed
pubmed-article:20960307pubmed:articleTitleIn vitro anti-viral activity of the total alkaloids from Tripterygium hypoglaucum against herpes simplex virus type 1.lld:pubmed
pubmed-article:20960307pubmed:affiliationBiomedicine Research and Development Center of Jinan University, Guangzhou 510632, China.lld:pubmed
pubmed-article:20960307pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20960307pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed