| pubmed-article:20960182 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20960182 | lifeskim:mentions | umls-concept:C1175743 | lld:lifeskim |
| pubmed-article:20960182 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:20960182 | lifeskim:mentions | umls-concept:C0015161 | lld:lifeskim |
| pubmed-article:20960182 | lifeskim:mentions | umls-concept:C0178774 | lld:lifeskim |
| pubmed-article:20960182 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:20960182 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:20960182 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:20960182 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:20960182 | pubmed:dateCreated | 2010-10-20 | lld:pubmed |
| pubmed-article:20960182 | pubmed:abstractText | In order to establish the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene expression. The recombinant plasmid of pTRE-Tight-SARS-N was constructed by using the plasmid p8S as the PCR template which contains a cDNA clone covering the nucleocapsid gene of SARS-CoV HKU-39449. Restriction enzymes digestion and sequence analysis indicated the recombinant plasmid of pTRE-Tight-SARS-N contained the nucleocapsid gene with the optimized nucleotide sequence which will improve the translation efficiency. Positive cell clones were selected by cotransfecting pTRE-Tight-SARS-N with the linear marker pPUR to BHK-21 Tet-on cells in the presence of puromycin. A set of double-stable eukaryotic cell lines (BHK-Tet-SARS-N) with inducible control of the SARS-CoV neucleocapsid gene expression was identified by using SDS-PAGE and Western-blot analysis. The expression of SARS-CoV nucleocapsid protein was tightly regulated by the varying concentration of doxcycline in the constructed double-stable cell line. The constructed BHK-Tet-SARS-N cell strains will facilitate the rescue of SARS-CoV in vitro and the further reverse genetic research of SARS-CoV. | lld:pubmed |
| pubmed-article:20960182 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20960182 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20960182 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20960182 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20960182 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20960182 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20960182 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20960182 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:20960182 | pubmed:issn | 1995-820X | lld:pubmed |
| pubmed-article:20960182 | pubmed:author | pubmed-author:RAYT WTW | lld:pubmed |
| pubmed-article:20960182 | pubmed:author | pubmed-author:SiddellStuart... | lld:pubmed |
| pubmed-article:20960182 | pubmed:author | pubmed-author:ZhuQing-YuQY | lld:pubmed |
| pubmed-article:20960182 | pubmed:author | pubmed-author:ChangGuo-HuiG... | lld:pubmed |
| pubmed-article:20960182 | pubmed:author | pubmed-author:WilsonMattM | lld:pubmed |
| pubmed-article:20960182 | pubmed:author | pubmed-author:DividsonAndre... | lld:pubmed |
| pubmed-article:20960182 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20960182 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:20960182 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20960182 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20960182 | pubmed:pagination | 361-8 | lld:pubmed |
| pubmed-article:20960182 | pubmed:meshHeading | pubmed-meshheading:20960182... | lld:pubmed |
| pubmed-article:20960182 | pubmed:meshHeading | pubmed-meshheading:20960182... | lld:pubmed |
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| pubmed-article:20960182 | pubmed:meshHeading | pubmed-meshheading:20960182... | lld:pubmed |
| pubmed-article:20960182 | pubmed:meshHeading | pubmed-meshheading:20960182... | lld:pubmed |
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| pubmed-article:20960182 | pubmed:meshHeading | pubmed-meshheading:20960182... | lld:pubmed |
| pubmed-article:20960182 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20960182 | pubmed:articleTitle | Establishment of the eukaryotic cell lines for inducible control of SARS-CoV nucleocapsid gene expression. | lld:pubmed |
| pubmed-article:20960182 | pubmed:affiliation | State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. changguohui999@yahoo.com.cn | lld:pubmed |
| pubmed-article:20960182 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20960182 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
