| pubmed-article:20839808 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20839808 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:20839808 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:20839808 | lifeskim:mentions | umls-concept:C1444748 | lld:lifeskim |
| pubmed-article:20839808 | lifeskim:mentions | umls-concept:C0032521 | lld:lifeskim |
| pubmed-article:20839808 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:20839808 | pubmed:issue | 39 | lld:pubmed |
| pubmed-article:20839808 | pubmed:dateCreated | 2010-9-30 | lld:pubmed |
| pubmed-article:20839808 | pubmed:abstractText | Despite the importance of protein-polymer bioconjugates, there is no general method for producing homogeneous recombinant protein that contains polymer initiators at defined sites. To address this deficiency, we designed the amino acid 4-(2'-bromoisobutyramido)phenylalanine (1) as an initiator in atom-transfer radical polymerization (ATRP) that would provide a stable linkage between the protein and growing polymer. We synthesized 1 and evolved a Methanococcus jannaschii tyrosyl-tRNA synthetase/tRNA(CUA) pair to genetically encode this initiator in response to an amber codon. To demonstrate the utility of this initiator, we produced green fluorescent protein (GFP) with 1 site-specifically incorporated on its surface (GFP-1). Purified GFP-1 was then used as an initiator under standard ATRP conditions with a monomer, oligo(ethylene oxide) monomethyl ether methacrylate, efficiently producing a polymer-GFP bioconjugate where the polymer is connected at our selected site on GFP. | lld:pubmed |
| pubmed-article:20839808 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20839808 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20839808 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20839808 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20839808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20839808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20839808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20839808 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20839808 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20839808 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:20839808 | pubmed:issn | 1520-5126 | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:HessKenneth... | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:Matyjaszewski... | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:MehlRyan ARA | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:AverickSaadya... | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:Miyake-Stoner... | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:PeelerJennife... | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:StokesAudrey... | lld:pubmed |
| pubmed-article:20839808 | pubmed:author | pubmed-author:WoodmanBradle... | lld:pubmed |
| pubmed-article:20839808 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20839808 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:20839808 | pubmed:volume | 132 | lld:pubmed |
| pubmed-article:20839808 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20839808 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20839808 | pubmed:pagination | 13575-7 | lld:pubmed |
| pubmed-article:20839808 | pubmed:meshHeading | pubmed-meshheading:20839808... | lld:pubmed |
| pubmed-article:20839808 | pubmed:meshHeading | pubmed-meshheading:20839808... | lld:pubmed |
| pubmed-article:20839808 | pubmed:meshHeading | pubmed-meshheading:20839808... | lld:pubmed |
| pubmed-article:20839808 | pubmed:meshHeading | pubmed-meshheading:20839808... | lld:pubmed |
| pubmed-article:20839808 | pubmed:meshHeading | pubmed-meshheading:20839808... | lld:pubmed |
| pubmed-article:20839808 | pubmed:meshHeading | pubmed-meshheading:20839808... | lld:pubmed |
| pubmed-article:20839808 | pubmed:meshHeading | pubmed-meshheading:20839808... | lld:pubmed |
| pubmed-article:20839808 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20839808 | pubmed:articleTitle | Genetically encoded initiator for polymer growth from proteins. | lld:pubmed |
| pubmed-article:20839808 | pubmed:affiliation | Department of Chemistry, Franklin & Marshall College, Lancaster, Pennsylvania 17604-3003, USA. | lld:pubmed |
| pubmed-article:20839808 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20839808 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:20839808 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
