| pubmed-article:20832435 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20832435 | lifeskim:mentions | umls-concept:C1094092 | lld:lifeskim |
| pubmed-article:20832435 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
| pubmed-article:20832435 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:20832435 | lifeskim:mentions | umls-concept:C0021585 | lld:lifeskim |
| pubmed-article:20832435 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:20832435 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:20832435 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:20832435 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:20832435 | pubmed:dateCreated | 2010-12-27 | lld:pubmed |
| pubmed-article:20832435 | pubmed:abstractText | VP37 protein of Broad bean wilt virus 2 (BBWV-2) is a multifunctional protein that binds single-strand nucleic acids, interacts with viral coat protein (CP) and potentiates the virus cell-to-cell movement in its host plant. In this study, tubule-like structures filled with virus-like particles were observed by Electron Microscopy in plasmodesmata in walls of Chenopodium quinoa leaf cells infected with BBWV-2. Immunogold labeling using VP37 protein specific antibody demonstrates that the VP37 is a component of the tubular structures. When VP37 was fused with the green fluorescent protein (VP37-GFP) and expressed in BY-2 protoplasts or in insect Tn cells, green fluorescent tubules of various lengths were produced, protruding from the surface of the expressing cells. These findings suggest that the movement of BBWV-2 between cells is mediated by the tubular structures that contain the VP37 protein, and the VP37 protein itself is capable of inducing these tubule-like structures in cells. Our results also suggest that the plant and insect cell factors involved in the tubule formation have conserved features. | lld:pubmed |
| pubmed-article:20832435 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20832435 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20832435 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20832435 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20832435 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20832435 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20832435 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20832435 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:20832435 | pubmed:issn | 1872-7492 | lld:pubmed |
| pubmed-article:20832435 | pubmed:author | pubmed-author:HongJianJ | lld:pubmed |
| pubmed-article:20832435 | pubmed:author | pubmed-author:ZhouXuepingX | lld:pubmed |
| pubmed-article:20832435 | pubmed:author | pubmed-author:LiuChengkeC | lld:pubmed |
| pubmed-article:20832435 | pubmed:author | pubmed-author:ZhangChuanxiC | lld:pubmed |
| pubmed-article:20832435 | pubmed:author | pubmed-author:YeLufeiL | lld:pubmed |
| pubmed-article:20832435 | pubmed:author | pubmed-author:LangGuojunG | lld:pubmed |
| pubmed-article:20832435 | pubmed:copyrightInfo | Copyright © 2010 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20832435 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20832435 | pubmed:volume | 155 | lld:pubmed |
| pubmed-article:20832435 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20832435 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20832435 | pubmed:pagination | 42-7 | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:meshHeading | pubmed-meshheading:20832435... | lld:pubmed |
| pubmed-article:20832435 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20832435 | pubmed:articleTitle | The VP37 protein of Broad bean wilt virus 2 induces tubule-like structures in both plant and insect cells. | lld:pubmed |
| pubmed-article:20832435 | pubmed:affiliation | State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China. | lld:pubmed |
| pubmed-article:20832435 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20832435 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20832435 | lld:entrezgene |
