| pubmed-article:20801471 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20801471 | lifeskim:mentions | umls-concept:C0078988 | lld:lifeskim |
| pubmed-article:20801471 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:20801471 | lifeskim:mentions | umls-concept:C0043100 | lld:lifeskim |
| pubmed-article:20801471 | lifeskim:mentions | umls-concept:C0039251 | lld:lifeskim |
| pubmed-article:20801471 | lifeskim:mentions | umls-concept:C0370215 | lld:lifeskim |
| pubmed-article:20801471 | lifeskim:mentions | umls-concept:C2347947 | lld:lifeskim |
| pubmed-article:20801471 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:20801471 | pubmed:dateCreated | 2010-9-20 | lld:pubmed |
| pubmed-article:20801471 | pubmed:abstractText | The low-molecular weight proteins (LMWPs) of Taenia solium metacestode (TsM) constituted pertinent serodiagnostic antigens for cysticercosis. We established a novel single-step purification of the LMWPs from TsM cyst fluid (CF). When the CF was precipitated with trichloroacetic acid/acetone mixture at the final concentrations of 5 and 50%, most LMWPs (ranging 7-38kDa) remained in the supernatant fraction. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot analyses revealed that the LMWPs consisted mainly with the previously reported subunit proteins of the 120- and 150-kDa antigen complexes. Serum samples from neurocysticercosis (NC) and other helminthic infections, as well as those from healthy controls were tested by both immunoblotting and micro-ELISA. In 135 cases of active stage NC patients, 132 cases (97.7%) showed positive reactions. Serum samples from other helminthic diseases (n=125) and healthy controls (n=100) exhibited no positive reactions except for cystic echinococcosis, of which 12% (3/25 cases) exhibited low levels of cross-reactivity. The LMWPs from different geographical regions (Korea and Mexico) showed diagnostic sensitivity and specificity of 97.7% and 98.7% against active stage NC. Our single-step separation method for the LMWPs provided excellent performance with easy applicability and high reproducibility, which has a great benefit for preparation of potent antigen in endemic areas. | lld:pubmed |
| pubmed-article:20801471 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20801471 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20801471 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20801471 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20801471 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20801471 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:20801471 | pubmed:issn | 1878-3503 | lld:pubmed |
| pubmed-article:20801471 | pubmed:author | pubmed-author:NawaYY | lld:pubmed |
| pubmed-article:20801471 | pubmed:author | pubmed-author:KongYY | lld:pubmed |
| pubmed-article:20801471 | pubmed:author | pubmed-author:YenF CFC | lld:pubmed |
| pubmed-article:20801471 | pubmed:author | pubmed-author:AabL ELE | lld:pubmed |
| pubmed-article:20801471 | pubmed:author | pubmed-author:BuiK LKL | lld:pubmed |
| pubmed-article:20801471 | pubmed:author | pubmed-author:Díaz-CamachoS... | lld:pubmed |
| pubmed-article:20801471 | pubmed:copyrightInfo | Copyright © 2010 Royal Society of Tropical Medicine and Hygiene. | lld:pubmed |
| pubmed-article:20801471 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20801471 | pubmed:volume | 104 | lld:pubmed |
| pubmed-article:20801471 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20801471 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20801471 | pubmed:pagination | 676-83 | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:meshHeading | pubmed-meshheading:20801471... | lld:pubmed |
| pubmed-article:20801471 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20801471 | pubmed:articleTitle | Serodiagnostic reliability of single-step enriched low-molecular weight proteins of Taenia solium metacestode of American and Asian isolates. | lld:pubmed |
| pubmed-article:20801471 | pubmed:affiliation | Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon 440-747, Korea. | lld:pubmed |
| pubmed-article:20801471 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20801471 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
