| pubmed-article:20708940 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20708940 | lifeskim:mentions | umls-concept:C1082344 | lld:lifeskim |
| pubmed-article:20708940 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:20708940 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:20708940 | lifeskim:mentions | umls-concept:C1155064 | lld:lifeskim |
| pubmed-article:20708940 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:20708940 | lifeskim:mentions | umls-concept:C1871232 | lld:lifeskim |
| pubmed-article:20708940 | lifeskim:mentions | umls-concept:C1101425 | lld:lifeskim |
| pubmed-article:20708940 | pubmed:issue | 18 | lld:pubmed |
| pubmed-article:20708940 | pubmed:dateCreated | 2010-9-6 | lld:pubmed |
| pubmed-article:20708940 | pubmed:abstractText | The preserved fungal species Antrodia camphorata has diverse health-promoting effects and has been popularly used in East Asia as a traditional herb. We isolated a volatile compound from the culture medium of A. camphorata and identified it as gamma-dodecalactone (gamma-DDL). Cytomic screening for immune-modulating activity revealed that gamma-DDL can activate human NK cells to express the early activation marker CD69. Further experiments showed that gamma-DDL not only can induce NK cells to express CD69 but also stimulate NK cells to secrete cytotoxic molecules (FasL and granzyme B) and Th1 cytokines (TNF-alpha and INF-gamma). Measuring the distribution of gamma-DDL in the subcellular compartments of NK cells revealed that gamma-DDL has been converted to 4-hydroxydodecanoic acid (an acyclic isomer of gamma-DDL) in a time-dependent manner in the cytoplasm. Synthetic (R,S)-4-hydroxydodecanoic acid activated NK cells to express CD69 mRNA within 10min, in contrast to gamma-DDL, which activated NK cells to express CD69 within 50min. This faster activation suggests that gamma-DDL has converted to 4-hydroxydodecanoic acid and to stimulate the NK cells to express CD69. Optically pure (R)-(+)-4-hydroxydodecanoic acid and (S)-(-)-4-hydroxydodecanoic acid were obtained via: (1) synthesis of its diastereomeric esters of (R,S)-4-hydroxydodecanoic (R)-(-)-2-phenylpropionate; (2) separation of diastereomers via preparative HPLC, and (3) subsequent hydrolysis of the obtained optical pure ester of (R)-(+)-4-hydroxydodecanoic acid (R)-(-)-2-phenylpropionate and (R)-(-)-4-hydroxydodecanoic acid (R)-(-)-2-phenylpropionate, respectively. Further assays of NK cells activation using each enantiomer showed that only the (R)-(+)-4-hydroxydodecanoic acid can activate NK cells. | lld:pubmed |
| pubmed-article:20708940 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20708940 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20708940 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20708940 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:20708940 | pubmed:issn | 1464-3391 | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:ChenShui-Tein... | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:ChangHwan-You... | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:ChienChichen... | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:ChenChia-Jung... | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:TsaiChia-CheC... | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:WuWan-HsunWH | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:ChaoLouis... | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:Vijaya... | lld:pubmed |
| pubmed-article:20708940 | pubmed:author | pubmed-author:HwangKent-Hao... | lld:pubmed |
| pubmed-article:20708940 | pubmed:copyrightInfo | Copyright (c) 2010 Elsevier Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:20708940 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20708940 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:20708940 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:20708940 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20708940 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20708940 | pubmed:pagination | 6896-904 | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
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| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:meshHeading | pubmed-meshheading:20708940... | lld:pubmed |
| pubmed-article:20708940 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20708940 | pubmed:articleTitle | Structure and functions of gamma-dodecalactone isolated from Antrodia camphorata for NK cell activation. | lld:pubmed |
| pubmed-article:20708940 | pubmed:affiliation | Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan. | lld:pubmed |
| pubmed-article:20708940 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | http://linkedlifedata.com/r... | pubmed-article:20708940 | lld:chembl |
