| pubmed-article:20706209 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20706209 | lifeskim:mentions | umls-concept:C0162610 | lld:lifeskim |
| pubmed-article:20706209 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:20706209 | pubmed:dateCreated | 2010-8-13 | lld:pubmed |
| pubmed-article:20706209 | pubmed:abstractText | Insulin/IGF-1 signaling controls metabolism, stress resistance and aging in Caenorhabditis elegans by regulating the activity of the DAF-16/FoxO transcription factor (TF). However, the function of DAF-16 and the topology of the transcriptional network that it crowns remain unclear. Using chromatin profiling by DNA adenine methyltransferase identification (DamID), we identified 907 genes that are bound by DAF-16. These were enriched for genes showing DAF-16-dependent upregulation in long-lived daf-2 insulin/IGF-1 receptor mutants (P=1.4e(-11)). Cross-referencing DAF-16 targets with these upregulated genes (daf-2 versus daf-16; daf-2) identified 65 genes that were DAF-16 regulatory targets. These 65 were enriched for signaling genes, including known determinants of longevity, but not for genes specifying somatic maintenance functions (e.g. detoxification, repair). This suggests that DAF-16 acts within a relatively small transcriptional subnetwork activating (but not suppressing) other regulators of stress resistance and aging, rather than directly regulating terminal effectors of longevity. For most genes bound by DAF-16::DAM, transcriptional regulation by DAF-16 was not detected, perhaps reflecting transcriptionally non-functional TF 'parking sites'. This study demonstrates the efficacy of DamID for chromatin profiling in C. elegans. | lld:pubmed |
| pubmed-article:20706209 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20706209 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20706209 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20706209 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20706209 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20706209 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:20706209 | pubmed:issn | 1744-4292 | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:VanfleterenJa... | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:ThorntonJanet... | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:Reece-HoyesJo... | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:GemsDavidD | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:HopeIan AIA | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:MatthijssensF... | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:McElweeJoshua... | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:TulletJennife... | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:SchusterEugen... | lld:pubmed |
| pubmed-article:20706209 | pubmed:author | pubmed-author:DoonanRyanR | lld:pubmed |
| pubmed-article:20706209 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20706209 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:20706209 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:20706209 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20706209 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20706209 | pubmed:pagination | 399 | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
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| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:meshHeading | pubmed-meshheading:20706209... | lld:pubmed |
| pubmed-article:20706209 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20706209 | pubmed:articleTitle | DamID in C. elegans reveals longevity-associated targets of DAF-16/FoxO. | lld:pubmed |
| pubmed-article:20706209 | pubmed:affiliation | Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, UK. e.schuster@ucl.ac.uk | lld:pubmed |
| pubmed-article:20706209 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20706209 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20706209 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:172981 | entrezgene:pubmed | pubmed-article:20706209 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20706209 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20706209 | lld:pubmed |
