| pubmed-article:2070573 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0086962 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0040132 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0700384 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0004368 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C0523376 | lld:lifeskim |
| pubmed-article:2070573 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2070573 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2070573 | pubmed:dateCreated | 1991-8-20 | lld:pubmed |
| pubmed-article:2070573 | pubmed:abstractText | Severe combined immunodeficient (SCID) mice were injected with peripheral blood mononuclear cells (PBMC) from normal individuals and 14 out of 18 had detectable serum human (h) IgG (maximum levels providing a mean +/- SEM 934 +/- 213 micrograms/ml) and IgM (253 +/- 93 micrograms/ml) at 3-6 weeks after transplantation. Serum human immunoglobulin levels were maximum 6-12 weeks after transplantation and declined to low levels over the subsequent 5 months. Human B cells constituted up to 10% and human T cells up to 40% of cells in the peripheral circulation and spleens of these animals 2-3 weeks after transplantation, PBMC, or intrathyroidal (IT) lymphocytes, from 6 patients with Graves' disease and high serum levels of thyroid autoantibodies were transplanted into 30 SCID mice (Graves' SCID-hu). Although serum human immunoglobulins were observed in only low amounts in the animals receiving IT lymphocytes (n = 4), increased levels of hIgG or hIgM were more easily detectable in 19 Graves' SCID-hu mice that received PBMC. The Graves' SCID-hu mice had significantly lower mean levels of hIgG and hIgM than those observed following transplantation of normal PBMC (mean maximum 328 +/- 113 and 32 +/- 21 micrograms/ml, respectively). Six of these 19 mice had detectable human autoantibody to thyroid peroxidase (TPO, as microsomal antigen) between 3 and 8 weeks after transplantation, with titers ranging from 0.05 to 0.39 (normal SCID-hu serum less than 0.02 ELISA Index). No abnormal thyroid hormone (T4 and T3) levels or thyroiditis was seen when compared to normal SCID-hu mice. Immunization of reconstituted SCID mice with recombinant immunoactive human TPO antigen failed to initiate anti-TPO in normal PBMC-treated mice nor did it increase the titer of human anti-TPO in the anti-TPO positive animals. In conclusion we successfully established human thyroid autoantibody secretion in the SCID-hu mouse and characterized the transient nature of the model. Further studies will be required to achieve successful antigen presentation in this system. | lld:pubmed |
| pubmed-article:2070573 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2070573 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2070573 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2070573 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2070573 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2070573 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2070573 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2070573 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2070573 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2070573 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2070573 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:2070573 | pubmed:issn | 0090-1229 | lld:pubmed |
| pubmed-article:2070573 | pubmed:author | pubmed-author:KimuraHH | lld:pubmed |
| pubmed-article:2070573 | pubmed:author | pubmed-author:MartinAA | lld:pubmed |
| pubmed-article:2070573 | pubmed:author | pubmed-author:DaviesT FTF | lld:pubmed |
| pubmed-article:2070573 | pubmed:author | pubmed-author:ShultzL DLD | lld:pubmed |
| pubmed-article:2070573 | pubmed:author | pubmed-author:FongPP | lld:pubmed |
| pubmed-article:2070573 | pubmed:author | pubmed-author:ThungSS | lld:pubmed |
| pubmed-article:2070573 | pubmed:author | pubmed-author:KendlerDD | lld:pubmed |
| pubmed-article:2070573 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2070573 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:2070573 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2070573 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2070573 | pubmed:pagination | 319-30 | lld:pubmed |
| pubmed-article:2070573 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:2070573 | pubmed:meshHeading | pubmed-meshheading:2070573-... | lld:pubmed |
| pubmed-article:2070573 | pubmed:meshHeading | pubmed-meshheading:2070573-... | lld:pubmed |
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| pubmed-article:2070573 | pubmed:meshHeading | pubmed-meshheading:2070573-... | lld:pubmed |
| pubmed-article:2070573 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:2070573 | pubmed:articleTitle | The SCID-hu mouse and thyroid autoimmunity: characterization of human thyroid autoantibody secretion. | lld:pubmed |
| pubmed-article:2070573 | pubmed:affiliation | Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029. | lld:pubmed |
| pubmed-article:2070573 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2070573 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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