| pubmed-article:20660015 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20660015 | lifeskim:mentions | umls-concept:C1687052 | lld:lifeskim |
| pubmed-article:20660015 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:20660015 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:20660015 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:20660015 | pubmed:dateCreated | 2010-12-2 | lld:pubmed |
| pubmed-article:20660015 | pubmed:abstractText | Small RNAs are well described in higher eukaryotes such as mammals and plants; however, knowledge in simple eukaryotes such as filamentous fungi is limited. In this study, we discovered and characterized methylguanosine-capped and polyadenylated small RNAs (CPA-sRNAs) by using differential RNA selection, full-length cDNA cloning and 454 transcriptome sequencing of the rice blast fungus Magnaporthe oryzae. This fungus causes blast, a devastating disease on rice, the principle food staple for over half the world's population. CPA-sRNAs mapped primarily to the transcription initiation and termination sites of protein-coding genes and were positively correlated with gene expression, particularly for highly expressed genes including those encoding ribosomal proteins. Numerous CPA-sRNAs also mapped to rRNAs, tRNAs, snRNAs, transposable elements and intergenic regions. Many other 454 sequence reads could not be mapped to the genome; however, inspection revealed evidence for non-template additions and chimeric sequences. CPA-sRNAs were independently confirmed using a high affinity variant of eIF-4E to capture 5'-methylguanosine-capped RNA followed by 3'-RACE sequencing. These results expand the repertoire of small RNAs in filamentous fungi. | lld:pubmed |
| pubmed-article:20660015 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20660015 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20660015 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20660015 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:20660015 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:YuZ PZP | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:BrownDouglas... | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:DeanRalph ARA | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:HagedornCurt... | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:MitchellThoma... | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:SailsberyJosh... | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:GowdaMalaliM | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:MengShaowuS | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:OhYeonyeeY | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:NelsonCassie... | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:NunesCristian... | lld:pubmed |
| pubmed-article:20660015 | pubmed:author | pubmed-author:XueMinfengM | lld:pubmed |
| pubmed-article:20660015 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20660015 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:20660015 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20660015 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20660015 | pubmed:pagination | 7558-69 | lld:pubmed |
| pubmed-article:20660015 | pubmed:dateRevised | 2011-8-24 | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
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| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:meshHeading | pubmed-meshheading:20660015... | lld:pubmed |
| pubmed-article:20660015 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20660015 | pubmed:articleTitle | Genome-wide characterization of methylguanosine-capped and polyadenylated small RNAs in the rice blast fungus Magnaporthe oryzae. | lld:pubmed |
| pubmed-article:20660015 | pubmed:affiliation | Fungal Genomics Laboratory, Center for Integrated Fungal Research, North Carolina State University, Raleigh, NC 27606, USA. | lld:pubmed |
| pubmed-article:20660015 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20660015 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20660015 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20660015 | lld:pubmed |
