| pubmed-article:20529823 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C0008059 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C0011854 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C0003209 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:20529823 | lifeskim:mentions | umls-concept:C1515999 | lld:lifeskim |
| pubmed-article:20529823 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:20529823 | pubmed:dateCreated | 2010-6-10 | lld:pubmed |
| pubmed-article:20529823 | pubmed:abstractText | Type 1 diabetes mellitus (T1DM) is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group) were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha), activatory molecules (OX40, 4-1BB) and elements of cytotoxicity (granzyme B, perforin 1) were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted. | lld:pubmed |
| pubmed-article:20529823 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20529823 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20529823 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20529823 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:20529823 | pubmed:issn | 1897-5631 | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:ChyczewskiLec... | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:Stasiak-Barmu... | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:KretowskiAdam... | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:GórskaMariaM | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:BossowskiArtu... | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:?uczy?skiW?od... | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:I?endoElzbiet... | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:Wawrusiewicz-... | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:KosJustynaJ | lld:pubmed |
| pubmed-article:20529823 | pubmed:author | pubmed-author:JuchniewiczAg... | lld:pubmed |
| pubmed-article:20529823 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20529823 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:20529823 | pubmed:volume | 48 | lld:pubmed |
| pubmed-article:20529823 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20529823 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20529823 | pubmed:pagination | 93-100 | lld:pubmed |
| pubmed-article:20529823 | pubmed:meshHeading | pubmed-meshheading:20529823... | lld:pubmed |
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| pubmed-article:20529823 | pubmed:meshHeading | pubmed-meshheading:20529823... | lld:pubmed |
| pubmed-article:20529823 | pubmed:meshHeading | pubmed-meshheading:20529823... | lld:pubmed |
| pubmed-article:20529823 | pubmed:meshHeading | pubmed-meshheading:20529823... | lld:pubmed |
| pubmed-article:20529823 | pubmed:meshHeading | pubmed-meshheading:20529823... | lld:pubmed |
| pubmed-article:20529823 | pubmed:meshHeading | pubmed-meshheading:20529823... | lld:pubmed |
| pubmed-article:20529823 | pubmed:meshHeading | pubmed-meshheading:20529823... | lld:pubmed |
| pubmed-article:20529823 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20529823 | pubmed:articleTitle | The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes. | lld:pubmed |
| pubmed-article:20529823 | pubmed:affiliation | Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Medical University of Bia?ystok, Bia?ystok, Poland. w.luczynski@wp.pl | lld:pubmed |
| pubmed-article:20529823 | pubmed:publicationType | Journal Article | lld:pubmed |
