| pubmed-article:20489210 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20489210 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
| pubmed-article:20489210 | lifeskim:mentions | umls-concept:C1524075 | lld:lifeskim |
| pubmed-article:20489210 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:20489210 | lifeskim:mentions | umls-concept:C0052276 | lld:lifeskim |
| pubmed-article:20489210 | pubmed:issue | 32 | lld:pubmed |
| pubmed-article:20489210 | pubmed:dateCreated | 2010-8-2 | lld:pubmed |
| pubmed-article:20489210 | pubmed:abstractText | Classical arabinogalactan proteins partially defined by type II O-Hyp-linked arabinogalactans (Hyp-AGs) are structural components of the plant extracellular matrix. Recently we described the structure of a small Hyp-AG putatively based on repetitive trigalactosyl subunits and suggested that AGs are less complex and varied than generally supposed. Here we describe three additional AGs with similar subunits. The Hyp-AGs were isolated from two different arabinogalactan protein fusion glycoproteins expressed in tobacco cells; that is, a 22-residue Hyp-AG and a 20-residue Hyp-AG, both isolated from interferon alpha2b-(Ser-Hyp)(20), and a 14-residue Hyp-AG isolated from (Ala-Hyp)(51)-green fluorescent protein. We used NMR spectroscopy to establish the molecular structure of these Hyp-AGs, which share common features: (i) a galactan main chain composed of two 1-->3 beta-linked trigalactosyl blocks linked by a beta-1-->6 bond; (ii) bifurcated side chains with Ara, Rha, GlcUA, and a Gal 6-linked to Gal-1 and Gal-2 of the main-chain trigalactosyl repeats; (iii) a common side chain structure composed of up to six residues, the largest consisting of an alpha-L-Araf-(1-->5)-alpha-L-Araf-(1-->3)-alpha-L-Araf-(1-->3- unit and an alpha-L-Rhap-(1-->4)-beta-D-GlcUAp-(1-->6)-unit, both linked to Gal. The conformational ensemble obtained by using nuclear Overhauser effect data in structure calculations revealed a galactan main chain with a reverse turn involving the beta-1-->6 link between the trigalactosyl blocks, yielding a moderately compact structure stabilized by H-bonds. | lld:pubmed |
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| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20489210 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20489210 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20489210 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:20489210 | pubmed:issn | 1083-351X | lld:pubmed |
| pubmed-article:20489210 | pubmed:author | pubmed-author:XuJianfengJ | lld:pubmed |
| pubmed-article:20489210 | pubmed:author | pubmed-author:TanLiL | lld:pubmed |
| pubmed-article:20489210 | pubmed:author | pubmed-author:QiuFengF | lld:pubmed |
| pubmed-article:20489210 | pubmed:author | pubmed-author:YuanChunhuaC | lld:pubmed |
| pubmed-article:20489210 | pubmed:author | pubmed-author:KieliszewskiM... | lld:pubmed |
| pubmed-article:20489210 | pubmed:author | pubmed-author:LamportDerek... | lld:pubmed |
| pubmed-article:20489210 | pubmed:author | pubmed-author:VarnaiPeterP | lld:pubmed |
| pubmed-article:20489210 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20489210 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:20489210 | pubmed:volume | 285 | lld:pubmed |
| pubmed-article:20489210 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20489210 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20489210 | pubmed:pagination | 24575-83 | lld:pubmed |
| pubmed-article:20489210 | pubmed:dateRevised | 2011-8-25 | lld:pubmed |
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| pubmed-article:20489210 | pubmed:meshHeading | pubmed-meshheading:20489210... | lld:pubmed |
| pubmed-article:20489210 | pubmed:meshHeading | pubmed-meshheading:20489210... | lld:pubmed |
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| pubmed-article:20489210 | pubmed:meshHeading | pubmed-meshheading:20489210... | lld:pubmed |
| pubmed-article:20489210 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20489210 | pubmed:articleTitle | Plant O-hydroxyproline arabinogalactans are composed of repeating trigalactosyl subunits with short bifurcated side chains. | lld:pubmed |
| pubmed-article:20489210 | pubmed:affiliation | Department of Chemistry and Biochemistry, Biochemistry Research Facility, Ohio University, Athens, Ohio 45701, USA. | lld:pubmed |
| pubmed-article:20489210 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20489210 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20489210 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
