| pubmed-article:20462755 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0026922 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0012611 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0205473 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0205296 | lld:lifeskim |
| pubmed-article:20462755 | lifeskim:mentions | umls-concept:C0027600 | lld:lifeskim |
| pubmed-article:20462755 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:20462755 | pubmed:dateCreated | 2010-5-21 | lld:pubmed |
| pubmed-article:20462755 | pubmed:abstractText | In order to generate substantial amounts of neoglycoconjugate needed for commercialization of diagnostic kits and high-throughput detection of leprosy, we developed a facile and high-yield synthesis of the corresponding disaccharide. Herein, the non-reducing disaccharide segment of phenolic glycolipid I from Mycobacterium leprae, O-(3,6-di-O-methyl-beta-D-glucopyranosyl)-(1-->4)-O-2,3-di-O-methyl-alpha-L-rhamnopyranose was synthesized by an improved procedure. The disaccharide was efficiently conjugated to bovine/human serum albumin, via acyl-azide intermediate, to form natural disaccharide-BSA/HSA neoglycoproteins that showed a high activity in serodiagnosis of leprosy. The disaccharide incorporated into the proteins was accurately measured by MALDI-TOF mass spectrometry. The serological activities of the neoglycoproteins against pooled human lepromatous leprosy sera were measured by ELISA and they were detectable at picogram amounts. | lld:pubmed |
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| pubmed-article:20462755 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20462755 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20462755 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20462755 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20462755 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20462755 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20462755 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20462755 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20462755 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20462755 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20462755 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:20462755 | pubmed:issn | 1464-3405 | lld:pubmed |
| pubmed-article:20462755 | pubmed:author | pubmed-author:ZhangJianJ | lld:pubmed |
| pubmed-article:20462755 | pubmed:author | pubmed-author:SpencerJohn... | lld:pubmed |
| pubmed-article:20462755 | pubmed:author | pubmed-author:BrennanPatric... | lld:pubmed |
| pubmed-article:20462755 | pubmed:author | pubmed-author:ChatterjeeDel... | lld:pubmed |
| pubmed-article:20462755 | pubmed:author | pubmed-author:LiavAvrahamA | lld:pubmed |
| pubmed-article:20462755 | pubmed:copyrightInfo | Copyright 2010 Elsevier Ltd. All rights reserved. | lld:pubmed |
| pubmed-article:20462755 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20462755 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:20462755 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:20462755 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20462755 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20462755 | pubmed:pagination | 3250-3 | lld:pubmed |
| pubmed-article:20462755 | pubmed:dateRevised | 2011-7-28 | lld:pubmed |
| pubmed-article:20462755 | pubmed:meshHeading | pubmed-meshheading:20462755... | lld:pubmed |
| pubmed-article:20462755 | pubmed:meshHeading | pubmed-meshheading:20462755... | lld:pubmed |
| pubmed-article:20462755 | pubmed:meshHeading | pubmed-meshheading:20462755... | lld:pubmed |
| pubmed-article:20462755 | pubmed:meshHeading | pubmed-meshheading:20462755... | lld:pubmed |
| pubmed-article:20462755 | pubmed:meshHeading | pubmed-meshheading:20462755... | lld:pubmed |
| pubmed-article:20462755 | pubmed:meshHeading | pubmed-meshheading:20462755... | lld:pubmed |
| pubmed-article:20462755 | pubmed:meshHeading | pubmed-meshheading:20462755... | lld:pubmed |
| pubmed-article:20462755 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20462755 | pubmed:articleTitle | A modified synthesis and serological evaluation of neoglycoproteins containing the natural disaccharide of PGL-I from Mycobacterium leprae. | lld:pubmed |
| pubmed-article:20462755 | pubmed:affiliation | Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA. jian.zhang@adatech.com | lld:pubmed |
| pubmed-article:20462755 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20462755 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:20462755 | lld:pubmed |
