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pubmed-article:20433741pubmed:abstractTextGenetic predisposition to scrapie in sheep is associated with several variations in the peptide sequence of the prion protein gene (PRNP). DNA-based tests for scoring PRNP codons are essential tools for eradicating scrapie and for evaluating rare alleles for increased resistance to disease. In addition to those associated with scrapie, there are dozens more PRNP polymorphisms that may occur in various flocks. If not accounted for, these sites may cause base-pair mismatching with oligonucleotides used in DNA testing. Thus, the fidelity of scrapie genetic testing is enhanced by knowing the position and frequency of PRNP polymorphisms in targeted flocks.lld:pubmed
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pubmed-article:20433741pubmed:authorpubmed-author:SmithTimothy...lld:pubmed
pubmed-article:20433741pubmed:authorpubmed-author:ClawsonMichae...lld:pubmed
pubmed-article:20433741pubmed:authorpubmed-author:HeatonMichael...lld:pubmed
pubmed-article:20433741pubmed:authorpubmed-author:LaegreidWilli...lld:pubmed
pubmed-article:20433741pubmed:authorpubmed-author:FrekingBrad...lld:pubmed
pubmed-article:20433741pubmed:authorpubmed-author:LeymasterKreg...lld:pubmed
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pubmed-article:20433741pubmed:dateRevised2010-9-30lld:pubmed
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pubmed-article:20433741pubmed:articleTitleOvine reference materials and assays for prion genetic testing.lld:pubmed
pubmed-article:20433741pubmed:affiliationUSDA, ARS, United States Meat Animal Research Center State Spur 18D, Clay Center, NE 68933, USA. mike.heaton@ars.usda.govlld:pubmed
pubmed-article:20433741pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20433741pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed