| pubmed-article:20374530 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C1326471 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C0008266 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C0813145 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C0439780 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C0243127 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C1549945 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C0220903 | lld:lifeskim |
| pubmed-article:20374530 | lifeskim:mentions | umls-concept:C0443331 | lld:lifeskim |
| pubmed-article:20374530 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:20374530 | pubmed:dateCreated | 2010-7-19 | lld:pubmed |
| pubmed-article:20374530 | pubmed:abstractText | A genetic screen in Arabidopsis was developed to explore the regulation of chloroplast protein import in vivo using two independent reporters representing housekeeping and photosynthetic pre-proteins. We first used 5-enolpyruvylshikimate 3-phosphate synthase (EPSP synthase*), a key enzyme in the shikimic acid pathway, with a mutation that confers tolerance to the herbicide glyphosate. Because the EPSP synthase* pre-protein must be imported for its function, the loss of glyphosate tolerance provided an initial indication of an import deficiency. Second, the fate of GFP fused to a ferredoxin transit peptide (FD5-GFP) was determined. A class of altered chloroplast import (aci) mutants showed both glyphosate sensitivity and FD5-GFP mislocalized to nuclei. aci2-1 was selected for further study. Yellow fluorescent protein (YFP) fused to the transit peptide of EPSP synthase* or the small subunit of Rubisco was not imported into chloroplasts, but also localized to nuclei during protoplast transient expression. Isolated aci2-1 chloroplasts showed a 50% reduction in pre-protein import efficiency in an in vitro assay. Mutants did not grow photoautotrophically on media without sucrose and were small and dark green in soil. aci2-1 and two alleles code for Moco-sulfurase, which activates the aldehyde oxidases required for the biosynthesis of the plant hormones abscisic acid (ABA) and indole-acetic acid (IAA) and controls purine nucleotide (ATP and GTP) turnover and nitrogen recycling via xanthine dehydrogenase. These enzyme activities were not detected in aci2-1. ABA, IAA and/or purine turnover may play previously unrecognized roles in the regulation of chloroplast protein import in response to developmental, metabolic and environmental cues. | lld:pubmed |
| pubmed-article:20374530 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20374530 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20374530 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20374530 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:20374530 | pubmed:issn | 1365-313X | lld:pubmed |
| pubmed-article:20374530 | pubmed:author | pubmed-author:ThompsonJenni... | lld:pubmed |
| pubmed-article:20374530 | pubmed:author | pubmed-author:LamppaGayle... | lld:pubmed |
| pubmed-article:20374530 | pubmed:author | pubmed-author:ZhongRongR | lld:pubmed |
| pubmed-article:20374530 | pubmed:author | pubmed-author:OttesenEricE | lld:pubmed |
| pubmed-article:20374530 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20374530 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:20374530 | pubmed:volume | 63 | lld:pubmed |
| pubmed-article:20374530 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20374530 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20374530 | pubmed:pagination | 44-59 | lld:pubmed |
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| pubmed-article:20374530 | pubmed:meshHeading | pubmed-meshheading:20374530... | lld:pubmed |
| pubmed-article:20374530 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20374530 | pubmed:articleTitle | A forward genetic screen to explore chloroplast protein import in vivo identifies Moco sulfurase, pivotal for ABA and IAA biosynthesis and purine turnover. | lld:pubmed |
| pubmed-article:20374530 | pubmed:affiliation | Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA. | lld:pubmed |
| pubmed-article:20374530 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20374530 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| entrez-gene:838224 | entrezgene:pubmed | pubmed-article:20374530 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20374530 | lld:entrezgene |
