| pubmed-article:20104580 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C0024400 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C0334094 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C0487602 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:20104580 | lifeskim:mentions | umls-concept:C2348480 | lld:lifeskim |
| pubmed-article:20104580 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:20104580 | pubmed:dateCreated | 2010-2-26 | lld:pubmed |
| pubmed-article:20104580 | pubmed:abstractText | The capacity for robust proliferation upon re-infection is a hallmark of adaptive immunity and the basis of vaccination. A widely used animal model for the study of human disease is the rhesus macaque (RM), where capacity for proliferation can be assessed ex vivo using carboxyfluorescein succinimidyl ester (CFSE)-based dilution assays. However, we show over the course of the standard ex vivo proliferation assay that CFSE-labeling at commonly used dye concentrations induces significant cell death, but that this phenomenon is dose-dependent. Here, we describe an alternative semiquantitative method for estimating T cell proliferative responses that avoids the putative biases associated with chemical modification. RM peripheral blood mononuclear cells were stimulated ex vivo with cognate peptides for 5 days, immunostained for intracellular Ki-67, and then analyzed by flow cytometry. We describe a gating strategy using Ki-67 and side light scatter, also a marker of blastogenesis, which correlates strongly with data from CFSE dilution. We show that this method is a valid tool for measuring RM antigen-specific cellular proliferation ex vivo and can be used as an alternative to CFSE dilution assays. | lld:pubmed |
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| pubmed-article:20104580 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20104580 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20104580 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20104580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20104580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20104580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20104580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20104580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20104580 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20104580 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:20104580 | pubmed:issn | 1552-4930 | lld:pubmed |
| pubmed-article:20104580 | pubmed:author | pubmed-author:MuthumaniKaru... | lld:pubmed |
| pubmed-article:20104580 | pubmed:author | pubmed-author:WeinerDavid... | lld:pubmed |
| pubmed-article:20104580 | pubmed:author | pubmed-author:ShedlockDevon... | lld:pubmed |
| pubmed-article:20104580 | pubmed:author | pubmed-author:FerraroBernad... | lld:pubmed |
| pubmed-article:20104580 | pubmed:author | pubmed-author:HokeyDavid... | lld:pubmed |
| pubmed-article:20104580 | pubmed:author | pubmed-author:MorrowMatthew... | lld:pubmed |
| pubmed-article:20104580 | pubmed:author | pubmed-author:TalbottKendra... | lld:pubmed |
| pubmed-article:20104580 | pubmed:copyrightInfo | (c) 2010 International Society for Advancement of Cytometry. | lld:pubmed |
| pubmed-article:20104580 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20104580 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:20104580 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20104580 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20104580 | pubmed:pagination | 275-84 | lld:pubmed |
| pubmed-article:20104580 | pubmed:dateRevised | 2011-8-3 | lld:pubmed |
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