| pubmed-article:19712768 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0036638 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0028429 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0020964 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0042196 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0041221 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0205148 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C1705099 | lld:lifeskim |
| pubmed-article:19712768 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:19712768 | pubmed:issue | 44 | lld:pubmed |
| pubmed-article:19712768 | pubmed:dateCreated | 2009-10-9 | lld:pubmed |
| pubmed-article:19712768 | pubmed:abstractText | Chagas' disease, caused by infection with the protozoan parasite Trypanosoma cruzi (T. cruzi), is intractable showing a high mortality rate, and the development of effective vaccines is much desired. To examine the efficacy of a new mode of recombinant viral vaccine, we constructed two non-transmissible Sendai viruses (rSeV/dF) encoding the full-length parasite antigen amastigote surface protein-2 (ASP2) or ASP2 fused with a mono-ubiquitin on its N-terminus (UASP2). C57BL/6 mice immunized intranasally with rSeV/dF expressing either ASP2 or UASP2 showed significantly suppressed parasitemia and could be protected from lethal T. cruzi challenge. Depletion of CD8(+) T cells around the time of infection with T. cruzi completely abolished this protection, confirming that acquired immunity against the infection of T. cruzi is dependent on CD8(+) T cells. We also demonstrated that the protective immunity correlated with higher secretion of interferon-gamma (IFN-gamma) by spleen cells on in vitro-specific or non-specific stimulation. Increased CTL activity was also confirmed by degranulation or CTL assays. Interestingly, the control virus, rSeV/dF-GFP, induced even a higher IFN-gamma production from spleen cells following non-specific but not specific stimulation in vitro, suggesting that SeV may also be a good adjuvant when used as a vaccine vehicle. Taking together, the current findings indicate that recombinant Sendai virus expressing the ASP2 or UASP2 antigens of T. cruzi are interesting candidates for the development of a new mode of recombinant viral vaccine against Chagas' disease. | lld:pubmed |
| pubmed-article:19712768 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19712768 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19712768 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19712768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19712768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19712768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19712768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19712768 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19712768 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19712768 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:19712768 | pubmed:issn | 1873-2518 | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:YonemitsuYosh... | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:HasegawaMamor... | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:HisaedaHajime... | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:HimenoKunisuk... | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:IshidaHidekaz... | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:YoshidaKumiK | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:DuanXuefengX | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:TetsutaniKohh... | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:ChouBinB | lld:pubmed |
| pubmed-article:19712768 | pubmed:author | pubmed-author:TanakaSakuraS | lld:pubmed |
| pubmed-article:19712768 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19712768 | pubmed:day | 19 | lld:pubmed |
| pubmed-article:19712768 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:19712768 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19712768 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19712768 | pubmed:pagination | 6154-9 | lld:pubmed |
| pubmed-article:19712768 | pubmed:meshHeading | pubmed-meshheading:19712768... | lld:pubmed |
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| pubmed-article:19712768 | pubmed:meshHeading | pubmed-meshheading:19712768... | lld:pubmed |
| pubmed-article:19712768 | pubmed:meshHeading | pubmed-meshheading:19712768... | lld:pubmed |
| pubmed-article:19712768 | pubmed:meshHeading | pubmed-meshheading:19712768... | lld:pubmed |
| pubmed-article:19712768 | pubmed:meshHeading | pubmed-meshheading:19712768... | lld:pubmed |
| pubmed-article:19712768 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19712768 | pubmed:articleTitle | Efficient protective immunity against Trypanosoma cruzi infection after nasal vaccination with recombinant Sendai virus vector expressing amastigote surface protein-2. | lld:pubmed |
| pubmed-article:19712768 | pubmed:affiliation | Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. | lld:pubmed |
| pubmed-article:19712768 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19712768 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
