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pubmed-article:19711217pubmed:abstractTextA rapid and simple detection method for tetrodotoxin (TTX) in urine and plasma of patients with puffer fish poisoning was developed using commercially pre-packed solid-phase extraction (SPE) cartridges (C18 and weak cation exchange columns) and subsequent analyses by HPLC with UV detection. The detection limit of the standard TTX, TTX-spiked urine and plasma samples were all 10 ng/ml and the average TTX recovery in urine and plasma samples after SPE were 90.3 +/- 4.0 and 87.1 +/- 2.9%, respectively. It was noticed that the creatinine-adjusted urinary TTX levels obtained within the first 24 h of presentation apparently correlated much better with the severity of poisoning than the urinary TTX concentration without adjusting for variations in concomitant creatinine excretion.lld:pubmed
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pubmed-article:19711217pubmed:authorpubmed-author:YuChun-FaiCFlld:pubmed
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pubmed-article:19711217pubmed:authorpubmed-author:YuChung-HimCHlld:pubmed
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pubmed-article:19711217pubmed:articleTitleRapid screening of tetrodotoxin in urine and plasma of patients with puffer fish poisoning by HPLC with creatinine correction.lld:pubmed
pubmed-article:19711217pubmed:affiliationEnvironmental Biotechnology Laboratory, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China. vincentyu@ymail.comlld:pubmed
pubmed-article:19711217pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19711217pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed