| pubmed-article:19574424 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19574424 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:19574424 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:19574424 | lifeskim:mentions | umls-concept:C1261322 | lld:lifeskim |
| pubmed-article:19574424 | lifeskim:mentions | umls-concept:C0023907 | lld:lifeskim |
| pubmed-article:19574424 | lifeskim:mentions | umls-concept:C0386983 | lld:lifeskim |
| pubmed-article:19574424 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:19574424 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19574424 | pubmed:dateCreated | 2009-7-27 | lld:pubmed |
| pubmed-article:19574424 | pubmed:abstractText | Glypicans are heparan sulfate proteoglycans that are bound to the cell surface by glycosylphosphatidylinositol. While six members of the glypican family are known in mammals, our study focused on glypican 3 (GPC3). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel syndrome, an X-linked disorder characterized by pre- and postnatal liver and other organ overgrowth. GPC3 is overexpressed in human hepatocellular carcinoma; however, its role in normal liver regeneration and hepatocyte proliferation is unknown. Here we investigated the role of GPC3 in hepatocyte proliferation. GPC3 mRNA and protein levels begin to increase 2 days after hepatectomy with peak expression levels by day 5. In hepatocyte cultures, GPC3 reaches a plateau when hepatocyte proliferation decreases. In vitro studies using Morpholino oligonucleotides showed that blocking GPC3 expression promoted hepatocyte growth. Yeast two-hybrid assays revealed that GPC3 interacts with CD81, a member of the tetraspanin family that is reported to be involved in hepatitis C virus infection and cell proliferation. We found that CD81 levels also increased 2 days after partial hepatectomy and toward the end of regeneration. Immunofluorescence showed that CD81 and GPC3 colocalize by 2 and 6 days after hepatectomy. Co-immunoprecipitation validated the interaction of GPC3 and CD81. Our results indicate that GPC3 may be a negative regulator of liver regeneration and hepatocyte proliferation, and that this regulation may involve CD81. | lld:pubmed |
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| pubmed-article:19574424 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19574424 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19574424 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:19574424 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19574424 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19574424 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:19574424 | pubmed:issn | 1525-2191 | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:LuoJian-HuaJH | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:Michalopoulos... | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:MarsWendy MWM | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:BowenWilliam... | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:ParanjpeShiri... | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:BellAaron WAW | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:LiuBowenB | lld:pubmed |
| pubmed-article:19574424 | pubmed:author | pubmed-author:YuYan-PingYP | lld:pubmed |
| pubmed-article:19574424 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19574424 | pubmed:volume | 175 | lld:pubmed |
| pubmed-article:19574424 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19574424 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19574424 | pubmed:pagination | 717-24 | lld:pubmed |
| pubmed-article:19574424 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:19574424 | pubmed:meshHeading | pubmed-meshheading:19574424... | lld:pubmed |
| pubmed-article:19574424 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19574424 | pubmed:articleTitle | Investigation of the role of glypican 3 in liver regeneration and hepatocyte proliferation. | lld:pubmed |
| pubmed-article:19574424 | pubmed:affiliation | Department of Pathology, University of Pittsburgh School of Medicine, S-410 Biomedical Science Tower, Pittsburgh, PA 15261, USA. | lld:pubmed |
| pubmed-article:19574424 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19574424 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19574424 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:25236 | entrezgene:pubmed | pubmed-article:19574424 | lld:entrezgene |
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