| pubmed-article:19541636 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0028584 | lld:lifeskim |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0014239 | lld:lifeskim |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0682537 | lld:lifeskim |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0017082 | lld:lifeskim |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0142957 | lld:lifeskim |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0040671 | lld:lifeskim |
| pubmed-article:19541636 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:19541636 | pubmed:issue | 26 | lld:pubmed |
| pubmed-article:19541636 | pubmed:dateCreated | 2009-7-1 | lld:pubmed |
| pubmed-article:19541636 | pubmed:abstractText | The inner membrane of the nuclear envelope (NE) was previously shown to contain a Na/Ca exchanger (NCX) tightly linked to GM1 ganglioside that mediates transfer of nucleoplasmic Ca(2+) to the NE lumen and constitutes a cytoprotective mechanism. This transfer was initially observed with isolated nuclei and is now demonstrated in living cells in relation to subcellular Ca(2+) dynamics. Four cell lines with varying expression of NCX and GM1 in the NE were transfected with cameleon-fluorescent Ca(2+) indicators genetically targeted to NE/endoplasmic reticulum (ER) and nucleoplasm to monitor [Ca(2+)](ne/er) and [Ca(2+)](n) respectively. Cytosolic Ca(2+) ([Ca(2+)](cyt)) was indicated with fura-2. Thapsigargin caused progressive loss of [Ca(2+)](ne/er), which was rapidly replaced on addition of extrinsic Ca(2+) to those cells containing fully functional NCX/GM1: differentiated NG108-15 and C6 cells. Reduced elevation of [Ca(2+)](ne/er) following thapsigargin depletion occurred in cells containing little or no GM1 in the NE: undifferentiated NG108-15 and NG-CR72 cells. No change in [Ca(2+)](ne/er) due to applied Ca(2+) was seen in Jurkat cells, which entirely lack NCX. Ca(2+) entry to NE/ER was also blocked by KB-R7943, inhibitor of NCX. [Ca(2+)](n) and [Ca(2+)](cyt) were elevated independent of [Ca(2+)](ne/er) and remained in approximate equilibrium with each other. Ca(2+) rise in the ER originated in the NE region and extended to the entire ER network. These results indicate the nuclear NCX/GM1 complex acts to gate Ca(2+) transfer from cytosol to ER, an alternate route to the sarcoplasmic/endoplasmic reticulum calcium ATPase pump. They also suggest a possible contributory mechanism for independent regulation of nuclear Ca(2+). | lld:pubmed |
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| pubmed-article:19541636 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19541636 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19541636 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19541636 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19541636 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19541636 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:19541636 | pubmed:author | pubmed-author:AHH | lld:pubmed |
| pubmed-article:19541636 | pubmed:author | pubmed-author:WuGushengG | lld:pubmed |
| pubmed-article:19541636 | pubmed:author | pubmed-author:LuZi-HuaZH | lld:pubmed |
| pubmed-article:19541636 | pubmed:author | pubmed-author:LedeenRobert... | lld:pubmed |
| pubmed-article:19541636 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19541636 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:19541636 | pubmed:volume | 106 | lld:pubmed |
| pubmed-article:19541636 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19541636 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19541636 | pubmed:pagination | 10829-34 | lld:pubmed |
| pubmed-article:19541636 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:19541636 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19541636 | pubmed:articleTitle | Sodium-calcium exchanger complexed with GM1 ganglioside in nuclear membrane transfers calcium from nucleoplasm to endoplasmic reticulum. | lld:pubmed |
| pubmed-article:19541636 | pubmed:affiliation | Department of Neurology and Neurosciences, New Jersey Medical School-University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA. | lld:pubmed |
| pubmed-article:19541636 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19541636 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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