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pubmed-article:19449044rdf:typepubmed:Citationlld:pubmed
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pubmed-article:19449044pubmed:dateCreated2009-10-2lld:pubmed
pubmed-article:19449044pubmed:abstractTextThe purpose of this study was to evaluate the possibility of using a semi-automated repetitive DNA sequences-based polymerase chain reaction (rep-PCR) for typing Pseudomonas aeruginosa isolates. rep-PCR profiles obtained by the DiversiLab system of 84 P. aeruginosa isolates from distinct epidemiological situations were obtained. rep-PCR groupings were in good agreement with the origin of these isolates. Linked rep-PCR profiles were observed for isolates recovered from a same family of cystic fibrosis (CF) patients, for the etiological agents of clustered cases of nosocomial infections, and for some isolates recovered from a same hospital room. rep-PCR and pulsed-field gel electrophoresis SpeI restricted genomic DNA (PFGE-SpeI) profiles were compared. In a few instances, rep-PCR revealed genetic divergences among isolates of a same group of PFGE-SpeI profiles. These divergences could reflect genetic drifts among closely related isolates, as illustrated by those observed between clinical and environmental isolates of a same group of PFGE-SpeI profiles. The interpretation of such differences will require further studies, but the rep-PCR analysis of P. aeruginosa diversity appeared to be an appropriate method to investigate infra-specific genetic relatedness.lld:pubmed
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pubmed-article:19449044pubmed:authorpubmed-author:RenaudF N RFNlld:pubmed
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pubmed-article:19449044pubmed:authorpubmed-author:MazoyerM-AMAlld:pubmed
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pubmed-article:19449044pubmed:volume28lld:pubmed
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pubmed-article:19449044pubmed:pagination1105-11lld:pubmed
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pubmed-article:19449044pubmed:year2009lld:pubmed
pubmed-article:19449044pubmed:articleTitleReliability of Pseudomonas aeruginosa semi-automated rep-PCR genotyping in various epidemiological situations.lld:pubmed
pubmed-article:19449044pubmed:affiliationUniversité de Lyon, Lyon, France. anne.jordheim@recherche.univ-lyon1.frlld:pubmed
pubmed-article:19449044pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19449044pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:19449044pubmed:publicationTypeEvaluation Studieslld:pubmed
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