| pubmed-article:19394293 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19394293 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
| pubmed-article:19394293 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:19394293 | lifeskim:mentions | umls-concept:C0598496 | lld:lifeskim |
| pubmed-article:19394293 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:19394293 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:19394293 | lifeskim:mentions | umls-concept:C0332261 | lld:lifeskim |
| pubmed-article:19394293 | lifeskim:mentions | umls-concept:C1504308 | lld:lifeskim |
| pubmed-article:19394293 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19394293 | pubmed:dateCreated | 2009-4-27 | lld:pubmed |
| pubmed-article:19394293 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19394293 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19394293 | pubmed:abstractText | RNA interference (RNAi) plays a pivotal role in the formation of heterochromatin at the fission yeast centromeres. The RNA-induced transcriptional silencing (RITS) complex, composed of heterochromatic small interfering RNAs (siRNAs), the siRNA-binding protein Ago1, the chromodomain protein Chp1, and the Ago1/Chp1-interacting protein Tas3, provides a physical tether between the RNAi and heterochromatin assembly pathways. Here, we report the structural and functional characterization of a C-terminal Tas3 alpha-helical motif (TAM), which self-associates into a helical polymer and is required for cis spreading of RITS in centromeric DNA regions. Site-directed mutations of key residues within the hydrophobic monomer-monomer interface disrupt Tas3-TAM polymeric self-association in vitro and result in loss of gene silencing, spreading of RITS, and a dramatic reduction in centromeric siRNAs in vivo. These results demonstrate that, in addition to the chromodomain of Chp1 and siRNA-loaded Ago1, Tas3 self-association is required for RITS spreading and efficient heterochromatic gene silencing at centromeric repeat regions. | lld:pubmed |
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| pubmed-article:19394293 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19394293 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19394293 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19394293 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19394293 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19394293 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19394293 | pubmed:issn | 1097-4164 | lld:pubmed |
| pubmed-article:19394293 | pubmed:author | pubmed-author:PatelDinshaw... | lld:pubmed |
| pubmed-article:19394293 | pubmed:author | pubmed-author:MoazedDaneshD | lld:pubmed |
| pubmed-article:19394293 | pubmed:author | pubmed-author:WalzThomasT | lld:pubmed |
| pubmed-article:19394293 | pubmed:author | pubmed-author:LiHaitaoH | lld:pubmed |
| pubmed-article:19394293 | pubmed:author | pubmed-author:MotamediMoham... | lld:pubmed |
| pubmed-article:19394293 | pubmed:author | pubmed-author:YipCalvin KCK | lld:pubmed |
| pubmed-article:19394293 | pubmed:author | pubmed-author:WangZhanxinZ | lld:pubmed |
| pubmed-article:19394293 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19394293 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:19394293 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:19394293 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19394293 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19394293 | pubmed:pagination | 155-67 | lld:pubmed |
| pubmed-article:19394293 | pubmed:dateRevised | 2011-9-26 | lld:pubmed |
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