| pubmed-article:19343524 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C0329120 | lld:lifeskim |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C0023884 | lld:lifeskim |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C0012860 | lld:lifeskim |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C0205178 | lld:lifeskim |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C0205390 | lld:lifeskim |
| pubmed-article:19343524 | lifeskim:mentions | umls-concept:C0005052 | lld:lifeskim |
| pubmed-article:19343524 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19343524 | pubmed:dateCreated | 2009-4-3 | lld:pubmed |
| pubmed-article:19343524 | pubmed:abstractText | In the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons benzo[a]pyrene (B[a]P) were investigated in the liver of Sparus aurata (sea bream). Sexually immature gilthead sea bream were treated by intraperitoneal injection of B[a]P (20 mg kg(-1)) for 6, 12, 24, and 48 h. B[a]P accumulation was quantified in sea bream liver by mean of gas phase chromatography (GPC-MS) after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity, as a phase I biotransformation parameter; (2) liver glutathione S-transferase (GST) activity as a phase II conjugation enzyme. DNA damage was assessed over time using the single-cell gel electrophoresis comet assay. B[a]P bioaccumulation in the liver resulted in a biphasic curve with an increasing uptake up to 5.55 +/- 0.67 microg g(-1) dry weight after only 6 h exposure and 4.67 +/- 0.68 microg g(-1) dry weight after 48 h exposure. EROD activity showed a nonsymmetrical bell-shaped kinetic with a maximum at 24 h and lower but significant activities at 12 and 48 h with respect to control animals. Hepatic GST activities were only significant after 48 h exposure. Comet assay showed an increase in liver cells DNA damage with a maximum after 48 h exposure reaching up to 12.17 % DNA in the tail. | lld:pubmed |
| pubmed-article:19343524 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19343524 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19343524 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19343524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19343524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19343524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19343524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19343524 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19343524 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:19343524 | pubmed:issn | 1573-5168 | lld:pubmed |
| pubmed-article:19343524 | pubmed:author | pubmed-author:NarbonneJ FJF | lld:pubmed |
| pubmed-article:19343524 | pubmed:author | pubmed-author:BoussettaHH | lld:pubmed |
| pubmed-article:19343524 | pubmed:author | pubmed-author:BanniMM | lld:pubmed |
| pubmed-article:19343524 | pubmed:author | pubmed-author:GuerbejHH | lld:pubmed |
| pubmed-article:19343524 | pubmed:author | pubmed-author:BouraouiZZ | lld:pubmed |
| pubmed-article:19343524 | pubmed:author | pubmed-author:GhediraJJ | lld:pubmed |
| pubmed-article:19343524 | pubmed:author | pubmed-author:ClerandeauCC | lld:pubmed |
| pubmed-article:19343524 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19343524 | pubmed:volume | 35 | lld:pubmed |
| pubmed-article:19343524 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19343524 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19343524 | pubmed:pagination | 293-9 | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:meshHeading | pubmed-meshheading:19343524... | lld:pubmed |
| pubmed-article:19343524 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19343524 | pubmed:articleTitle | Acute effects of benzo[a]pyrene on liver phase I and II enzymes, and DNA damage on sea bream Sparus aurata. | lld:pubmed |
| pubmed-article:19343524 | pubmed:affiliation | Laboratoire de Biochimie et de Toxicologie Environnementale, Institut Supérieur Agronomique, Chott-Mariem, Sousse, Tunisia. m_banni@yahoo.fr | lld:pubmed |
| pubmed-article:19343524 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19343524 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
