| pubmed-article:19321421 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19321421 | lifeskim:mentions | umls-concept:C0085356 | lld:lifeskim |
| pubmed-article:19321421 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:19321421 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:19321421 | lifeskim:mentions | umls-concept:C1510827 | lld:lifeskim |
| pubmed-article:19321421 | lifeskim:mentions | umls-concept:C0547047 | lld:lifeskim |
| pubmed-article:19321421 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:19321421 | pubmed:dateCreated | 2009-4-8 | lld:pubmed |
| pubmed-article:19321421 | pubmed:abstractText | The CO(2)-concentrating mechanism (CCM) of Chlamydomonas reinhardtii and other microalgal species is essential for photosynthetic growth in most natural settings. A great deal has been learned regarding the CCM in cyanobacteria, including identification of inorganic carbon (Ci; CO(2) and HCO(3)(-)) transporters; however, specific knowledge of analogous transporters has remained elusive in eukaryotic microalgae such as C. reinhardtii. Here we investigated whether the limiting-CO(2)-inducible, putative ABC-type transporter HLA3 might function as a HCO(3)(-) transporter by evaluating the effect of pH on growth, photosynthetic Ci affinity, and [(14)C]-Ci uptake in very low CO(2) conditions following RNA interference (RNAi) knockdown of HLA3 mRNA levels in wild-type and mutant cells. Although knockdown of HLA3 mRNA alone resulted in only modest but high-pH-dependent decreases in photosynthetic Ci affinity and Ci uptake, the combination of nearly complete knockdown of HLA3 mRNA with mutations in LCIB (which encodes limiting-Ci-inducible plastid-localized protein required for normal Ci uptake or accumulation in low-CO(2) conditions) and/or simultaneous, apparently off-target knockdown of LCIA mRNA (which encodes limiting-Ci-inducible plastid envelope protein reported to transport HCO(3)(-)) resulted in dramatic decreases in growth, Ci uptake, and photosynthetic Ci affinity, especially at pH 9, at which HCO(3)(-) is the predominant form of available Ci. Collectively, the data presented here provide compelling evidence that HLA3 is directly or indirectly involved in HCO(3)(-) transport, along with additional evidence supporting a role for LCIA in chloroplast envelope HCO(3)(-) transport and a role for LCIB in chloroplast Ci accumulation. | lld:pubmed |
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| pubmed-article:19321421 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19321421 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19321421 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19321421 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19321421 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19321421 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:19321421 | pubmed:author | pubmed-author:HorkenKempton... | lld:pubmed |
| pubmed-article:19321421 | pubmed:author | pubmed-author:WeeksDonald... | lld:pubmed |
| pubmed-article:19321421 | pubmed:author | pubmed-author:SpaldingMarti... | lld:pubmed |
| pubmed-article:19321421 | pubmed:author | pubmed-author:MillerAmy RAR | lld:pubmed |
| pubmed-article:19321421 | pubmed:author | pubmed-author:DuanmuDeqiang... | lld:pubmed |
| pubmed-article:19321421 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19321421 | pubmed:day | 7 | lld:pubmed |
| pubmed-article:19321421 | pubmed:volume | 106 | lld:pubmed |
| pubmed-article:19321421 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19321421 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19321421 | pubmed:pagination | 5990-5 | lld:pubmed |
| pubmed-article:19321421 | pubmed:dateRevised | 2010-9-23 | lld:pubmed |
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| pubmed-article:19321421 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19321421 | pubmed:articleTitle | Knockdown of limiting-CO2-induced gene HLA3 decreases HCO3- transport and photosynthetic Ci affinity in Chlamydomonas reinhardtii. | lld:pubmed |
| pubmed-article:19321421 | pubmed:affiliation | Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA. | lld:pubmed |
| pubmed-article:19321421 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19321421 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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