| pubmed-article:19258281 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C0003732 | lld:lifeskim |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C0086382 | lld:lifeskim |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C0023779 | lld:lifeskim |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C0577559 | lld:lifeskim |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C0205160 | lld:lifeskim |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C1446409 | lld:lifeskim |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C0016126 | lld:lifeskim |
| pubmed-article:19258281 | lifeskim:mentions | umls-concept:C0075804 | lld:lifeskim |
| pubmed-article:19258281 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:19258281 | pubmed:dateCreated | 2009-6-11 | lld:pubmed |
| pubmed-article:19258281 | pubmed:abstractText | Lipids from the extremely halophilic Archaea, Haloarcula marismortui, contain abundant phytanyl diether phospholipids, namely archaetidic acid (AA), archaetidylglycerol (AG), archaetidylglycerosulfate (AGS), with mainly archaetidylglycerophosphate methyl ester (AGP-Me). These were accompanied by a triglycosyl archaeol (TGA), lacking characteristic sulfate groups. Tandem-mass spectrometry was employed to provide fingerprints for identifying these known lipids, as well as small amounts of unsaturated phospholipids. These contained 3 and 6 double bonds in their archaeol moiety, suggested by negative tandem-MS of intact phospholipids, as indicated by differences between their pseudo-molecular ion and specific fragment ions designated as pi(2). The core ether lipids were confirmed by electrospray ionization mass spectrometry (ESI-MS) as 2,3-di-O-phytanyl-sn-glycerol (C20, C20), which gave rise to a precursor-ion at m/z 660 [M+Li](+), and its fragment ion at m/z 379 [M+Li](+), consistent with mono-O-phytanyl-glycerol. Furthermore, lithiated ions at m/z 654 (MS(1)), 379 (MS(2)) and m/z 648 (MS(1)), 373 (MS(2)), combined with (1)H/(13)C NMR chemical shifts at delta 5.31-121.6 (C2/2'-H2/2'), 5.08-124.9 (C6/6'-H6/6') and 5.10-126.0 (10/10'-H10/10') confirmed the presence of unsaturated homologs of archaeol. We carried out a comprehensive study on the lipids present in cells of H. marismortui. We used positive and negative ESI-MS with tandem-MS, which served as a fingerprint analysis for identifying the majority of component lipids. | lld:pubmed |
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| pubmed-article:19258281 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19258281 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19258281 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19258281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19258281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19258281 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19258281 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:19258281 | pubmed:issn | 0022-2275 | lld:pubmed |
| pubmed-article:19258281 | pubmed:author | pubmed-author:SassakiGuilhe... | lld:pubmed |
| pubmed-article:19258281 | pubmed:author | pubmed-author:GorinPhilip... | lld:pubmed |
| pubmed-article:19258281 | pubmed:author | pubmed-author:IacominiMarce... | lld:pubmed |
| pubmed-article:19258281 | pubmed:author | pubmed-author:de... | lld:pubmed |
| pubmed-article:19258281 | pubmed:author | pubmed-author:Müller-Santos... | lld:pubmed |
| pubmed-article:19258281 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19258281 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:19258281 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19258281 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19258281 | pubmed:pagination | 1363-73 | lld:pubmed |
| pubmed-article:19258281 | pubmed:dateRevised | 2010-9-22 | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:meshHeading | pubmed-meshheading:19258281... | lld:pubmed |
| pubmed-article:19258281 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19258281 | pubmed:articleTitle | Positive and negative tandem mass spectrometric fingerprints of lipids from the halophilic Archaea Haloarcula marismortui. | lld:pubmed |
| pubmed-article:19258281 | pubmed:affiliation | Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba-PR, Brazil. | lld:pubmed |
| pubmed-article:19258281 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19258281 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
