| pubmed-article:19202055 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C0034865 | lld:lifeskim |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C1273518 | lld:lifeskim |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C1721094 | lld:lifeskim |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C1421876 | lld:lifeskim |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C2248928 | lld:lifeskim |
| pubmed-article:19202055 | lifeskim:mentions | umls-concept:C1707271 | lld:lifeskim |
| pubmed-article:19202055 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:19202055 | pubmed:dateCreated | 2009-2-25 | lld:pubmed |
| pubmed-article:19202055 | pubmed:abstractText | Activation-induced cytidine deaminase (AID) is an essential factor for the class switch recombination (CSR) and somatic hypermutation (SHM) of Ig genes. CSR and SHM are initiated by AID-induced DNA breaks in the S and V regions, respectively. Because truncation or frame-shift mutations at the carboxyl (C)-terminus of AID abolishes CSR but not SHM, the C-terminal region of AID likely is required for the targeting of DNA breaks in the S region. To test this hypothesis, we determined the precise location and relative amounts of AID-induced DNA cleavage using an in situ DNA end-labeling method. We established CH12F3-2 cell transfectants expressing the estrogen receptor (ER) fused with wild-type (WT) AID or a deletion mutant lacking the C-terminal 16 aa, JP8Bdel. We found that AID-ER, but not JP8Bdel-ER, caused a CSR to IgA from the addition of 4-hydroxy tamoxifen. In contrast, both WT AID and JP8Bdel induced DNA breaks in both the V and S regions. In addition, JP8Bdel enhanced c-myc/IgH translocations. Our findings indicate that the C-terminal domain of AID is not required for S-region DNA breaks but is required for S-region recombination after DNA cleavage. Therefore, AID does not distinguish between the V and S regions for cleavage, but carries another function specific to CSR. | lld:pubmed |
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| pubmed-article:19202055 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19202055 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19202055 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19202055 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19202055 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19202055 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19202055 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:HonjoTasukuT | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:WeiMinM | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:NagaokaHitosh... | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:WangJishuJ | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:ShinkuraReiko... | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:DoiTomomitsuT | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:ItoSatomiS | lld:pubmed |
| pubmed-article:19202055 | pubmed:author | pubmed-author:KatoLuciaL | lld:pubmed |
| pubmed-article:19202055 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19202055 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:19202055 | pubmed:volume | 106 | lld:pubmed |
| pubmed-article:19202055 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19202055 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19202055 | pubmed:pagination | 2758-63 | lld:pubmed |
| pubmed-article:19202055 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:19202055 | pubmed:meshHeading | pubmed-meshheading:19202055... | lld:pubmed |
| pubmed-article:19202055 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19202055 | pubmed:articleTitle | The C-terminal region of activation-induced cytidine deaminase is responsible for a recombination function other than DNA cleavage in class switch recombination. | lld:pubmed |
| pubmed-article:19202055 | pubmed:affiliation | Department of Immunology and Genomic Medicine, Graduate School of Medicine, Kyoto University, Yoshida Sakyo-ku, Kyoto 606-8501, Japan. | lld:pubmed |
| pubmed-article:19202055 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19202055 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:11628 | entrezgene:pubmed | pubmed-article:19202055 | lld:entrezgene |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19202055 | lld:pubmed |
