| pubmed-article:1917521 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C0023823 | lld:lifeskim |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C0023884 | lld:lifeskim |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C1323371 | lld:lifeskim |
| pubmed-article:1917521 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:1917521 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:1917521 | pubmed:dateCreated | 1991-11-21 | lld:pubmed |
| pubmed-article:1917521 | pubmed:abstractText | Lipoproteins were isolated either by immunoaffinity chromatography (LDL and VLDL) or ultracentrifugation (LDL). Purified lipoproteins were labeled with 123I using either Iodogen or iodine-monochloride (IC1) each followed by purification with gel-chromatography or dialysis (total of 4 combinations). Lipoprotein-concentrations of 0.1-6 micrograms protein/mL were used for direct binding assays investigating the specific binding of labeled lipoproteins (in the presence of a 50-fold excess of unlabeled lipoproteins) to human liver apo-B, E-receptors. In separate experiments displacement of bound 123I-lipoproteins (labeled by the methods mentioned) by unlabeled ones was studied. The binding capacities estimated by Scatchard analysis were similar to each other (141-163 ng protein bound/mg liver plasma membrane protein) independent from the method used for isolation and labeling. Also the affinity constants were very similar and ranged from 0.9 to 1.7 micrograms protein/L. It is concluded that immunoaffinity chromatography or ultracentrifugation for isolation of lipoproteins and the Iodogen or IC1-method for radiolabeling can be recommended to be equally good for in vitro receptor investigation. | lld:pubmed |
| pubmed-article:1917521 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1917521 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917521 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1917521 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917521 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917521 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917521 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1917521 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1917521 | pubmed:issn | 0883-2897 | lld:pubmed |
| pubmed-article:1917521 | pubmed:author | pubmed-author:SinzingerHH | lld:pubmed |
| pubmed-article:1917521 | pubmed:author | pubmed-author:AngelbergerPP | lld:pubmed |
| pubmed-article:1917521 | pubmed:author | pubmed-author:MolinariEE | lld:pubmed |
| pubmed-article:1917521 | pubmed:author | pubmed-author:VirgoliniII | lld:pubmed |
| pubmed-article:1917521 | pubmed:author | pubmed-author:PidlichJJ | lld:pubmed |
| pubmed-article:1917521 | pubmed:author | pubmed-author:RoR SRS | lld:pubmed |
| pubmed-article:1917521 | pubmed:author | pubmed-author:LupattelliGG | lld:pubmed |
| pubmed-article:1917521 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1917521 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:1917521 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1917521 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1917521 | pubmed:pagination | 513-7 | lld:pubmed |
| pubmed-article:1917521 | pubmed:dateRevised | 2008-2-21 | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:meshHeading | pubmed-meshheading:1917521-... | lld:pubmed |
| pubmed-article:1917521 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1917521 | pubmed:articleTitle | Comparison of different methods for LDL isolation and radioiodination on liver LDL receptor binding in vitro. | lld:pubmed |
| pubmed-article:1917521 | pubmed:affiliation | Atherosclerosis Research Group (ATK), Austrian Academy of Sciences. | lld:pubmed |
| pubmed-article:1917521 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1917521 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:1917521 | pubmed:publicationType | In Vitro | lld:pubmed |
