| pubmed-article:19128033 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19128033 | lifeskim:mentions | umls-concept:C0221908 | lld:lifeskim |
| pubmed-article:19128033 | lifeskim:mentions | umls-concept:C0242358 | lld:lifeskim |
| pubmed-article:19128033 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:19128033 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:19128033 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:19128033 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19128033 | pubmed:dateCreated | 2009-1-8 | lld:pubmed |
| pubmed-article:19128033 | pubmed:abstractText | Interferon-gamma (IFN-gamma) is an important proinflammatory cytokine that plays a central role in the intestinal inflammatory process of inflammatory bowel disease. IFN-gamma induced disturbance of the intestinal epithelial tight junction (TJ) barrier has been postulated to be an important mechanism contributing to intestinal inflammation. The intracellular mechanisms that mediate the IFN-gamma induced increase in intestinal TJ permeability remain unclear. The aim of this study was to examine the role of the phosphatidylinositol 3-kinase (PI3-K) pathway in the regulation of the IFN-gamma induced increase in intestinal TJ permeability using the T84 intestinal epithelial cell line. IFN-gamma caused an increase in T84 intestinal epithelial TJ permeability and depletion of TJ protein, occludin. The IFN-gamma induced increase in TJ permeability and alteration in occludin protein was associated with rapid activation of PI3-K; and inhibition of PI3-K activation prevented the IFN-gamma induced effects. IFN-gamma also caused a delayed but more prolonged activation of nuclear factor-kappaB (NF-kappaB); inhibition of NF-kappaB also prevented the increase in T84 TJ permeability and alteration in occludin expression. The IFN-gamma induced activation of NF-kappaB was mediated by a cross-talk with PI3-K pathway. In conclusion, the IFN-gamma induced increase in T84 TJ permeability and alteration in occludin protein expression were mediated by the PI3-K pathway. These results show for the first time that the IFN-gamma modulation of TJ protein and TJ barrier function is regulated by a cross-talk between PI3-K and NF-kappaB pathways. | lld:pubmed |
| pubmed-article:19128033 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19128033 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19128033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19128033 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19128033 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19128033 | pubmed:issn | 1557-7465 | lld:pubmed |
| pubmed-article:19128033 | pubmed:author | pubmed-author:KennedyJohn... | lld:pubmed |
| pubmed-article:19128033 | pubmed:author | pubmed-author:MaThomas YTY | lld:pubmed |
| pubmed-article:19128033 | pubmed:author | pubmed-author:BoivinMichel... | lld:pubmed |
| pubmed-article:19128033 | pubmed:author | pubmed-author:BradleyAngela... | lld:pubmed |
| pubmed-article:19128033 | pubmed:author | pubmed-author:RoyPraveen... | lld:pubmed |
| pubmed-article:19128033 | pubmed:author | pubmed-author:RihaniTuhamaT | lld:pubmed |
| pubmed-article:19128033 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19128033 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:19128033 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19128033 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19128033 | pubmed:pagination | 45-54 | lld:pubmed |
| pubmed-article:19128033 | pubmed:dateRevised | 2011-3-1 | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:meshHeading | pubmed-meshheading:19128033... | lld:pubmed |
| pubmed-article:19128033 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19128033 | pubmed:articleTitle | Mechanism of interferon-gamma-induced increase in T84 intestinal epithelial tight junction. | lld:pubmed |
| pubmed-article:19128033 | pubmed:affiliation | Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131-5271, USA. | lld:pubmed |
| pubmed-article:19128033 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19128033 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:19128033 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19128033 | lld:pubmed |
